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4.2.99.20: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

This is an abbreviated version!
For detailed information about 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, go to the full flat file.

Word Map on EC 4.2.99.20

Reaction

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate
=
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate
+
pyruvate

Synonyms

(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, EC 2.5.1.64, MenH, SHCHC, SHCHC synthase, YfbB

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.99 Other carbon-oxygen lyases
                4.2.99.20 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Engineering

Engineering on EC 4.2.99.20 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D128A
-
conserved among MenH proteins
D210A
F153A
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
H232A
H232K
-
part of the catalytically essential triad
K212A
-
conserved among MenH proteins
R124A
-
conserved among MenH proteins
R168A
-
conserved among MenH proteins
R90A
-
conserved among MenH proteins
V152A
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
V152G
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
V152G/F153A
inactive
W147A
-
conserved among MenH proteins
W147A/Y148A
inactive
W147F
-
conserved among MenH proteins
Y148A
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
Y148F
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
Y85F
-
conserved among MenH proteins