4.2.99.18: DNA-(apurinic or apyrimidinic site) lyase

This is an abbreviated version!
For detailed information about DNA-(apurinic or apyrimidinic site) lyase, go to the full flat file.

Reaction

the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate =

Synonyms

8-oxoG DNA glycosylase, abasic (AP)-endonuclease, abasic endonuclease, AdAPE1/Ref-1, Af0371, Afogg enzyme, ALKBH1, AP 1, AP DNA endonuclease 1, AP Dnase, AP endo, AP endonuclease, AP endonuclease 1, AP endonuclease Ape1, AP endonuclease Class I, AP endonuclease I, AP endonuclease VI, AP lyase, AP site-DNA 5'-phosphomonoester-lyase, AP-endonuclease, AP-endonuclease 1, AP-endonuclease 2, AP-endonuclease/3'-5'exodeoxyribonuclease, AP-lyase, Ap1, Ap2, Ape, APE-1, APE/Ref-1, APE1, APE1/Ref-1, Ape1L, Ape2, APEN, APEX nuclease, APEX1, APLM, APN, APN-1, APN1, apurinic DNA endonuclease, apurinic endodeoxyribonuclease, apurinic endonuclease, apurinic endonuclease 1, apurinic-apyrimidinic DNA endonuclease, apurinic-apyrimidinic endodeoxyribonuclease, apurinic-apyrimidinic endonuclease, apurinic-apyrimidinic endonuclease 1, apurinic-apyrimidinic endonuclease I, apurinic/apyrimidic endonuclease 1, apurinic/Apyrimidinic (AP) endonuclease 1, apurinic/apyrimidinic AP endonuclease, apurinic/apyrimidinic DNA endonuclease 1, apurinic/apyrimidinic endonuclease, apurinic/apyrimidinic endonuclease 1, apurinic/apyrimidinic endonuclease 1/redox factor-1, apurinic/apyrimidinic endonuclease 2, apurinic/apyrimidinic endonuclease APE1, apurinic/apyrimidinic endonuclease-1, apurinic/apyrimidinic endonuclease-1/Redox factor-1, apurinic/apyrimidinic endonuclease/redox effector factor-1, Apurinic/apyrimidinic endonuclease1/redox factor 1, apurinic/apyrimidinic endonuclease1/redox factor-1, apurinic/apyrimidinic lyase, apurinic/apyrimidinic site lyase, apurinic/apyrimidinic specific endonuclease, apurinic/apyrimidinic-endonuclease, apyrimidinic endonuclease, archaeal GO glycosylase, BAP1, class II AP endonuclease LMAP, class II apurinic/apyrimidinic(AP)-endonuclease, class-II AP-endonuclease, deoxyribonuclease (apurinic or apyrimidinic), DNA lyase, DNA polymerase X, DNA-(apurinic or apyrimidinic site) lyase, E. coli endonuclease III, EC 3.1.25.2, EcoNth, Endo III, endodeoxyribonuclease, endodeoxyribonuclease III, EndoIV, endonuclease III, endonuclease IV, endonuclease VI, endonuclease VIII, Escherichia coli endonuclease III, EXO-3, ExoA, ExoA type AP endonuclease, ExoIII, HAP-1, HAP1, HAP1h, hAPE, hAPE1, hNTH, hNTH1, hOgg1 protein, HpXth, human apurinic/apyrimidinic endonuclease, human apurinic/apyrimidinic endonuclease 1, Kae1, KsgA, LplExo, Micrococcus luteus UV endonuclease, MJ0724, mjOgg, MMH, More, mtAPE, MtbXthA, multi-functional apyrimidinic endonuclease1/redox factor-1, N-glycosylase AP lyase, N-glycosylase apurinic/apyrimidinic lyase, NapE, Nei, Nfo, Ntg1, Ntg1p, Ntg2, Ntg2p, Nth, NTH1, nuclease SmnA, nuclease, apurinic endodeoxyribo-, nuclease, apurinic-apyrimidinic endodeoxyribo-, nuclease, endodeoxyribo-, III, Ogg2, oxoG DNA glycosylase, Pa-AGOG, Pa-AGOG DNA glycosylase, PAE2237, PaKae1, PALF, pE296R, PF0258, phage-T4 UV endonuclease, redox effector factor 1, redox factor-1, redox factor-1:Ref-1, Ref-1, REF-1 protein, Ref1, SisExoIII, Smx nuclease, TvoExo, UL30, UV endo V, UV endonuclease, UV endonuclease V, X-ray endonuclease III, XTH, xthA, ZAP1, zApe

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.99 Other carbon-oxygen lyases

                4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase

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Results	in table
34	Activating Compound
96969	AA Sequence
31	Application
2	CAS Registry Number
89	Cloned(Commentary)
16	Crystallization (Commentary)
2167	Disease/ Diagnostics
6	Expression
98	General Information
5	General Stability
118	IC50 Value
386	Inhibitors
8	kcat/KM [mM/s]
91	Ki Value [mM]
164	KM Value [mM]
85	Localization
171	Metals/Ions
83	Molecular Weight [Da]
219	Natural Substrates/ Products (Substrates)
308	Organism
419	PDB ID
88	pH Optimum
15	pH Range
1	pH Stability
6	pI Value
23	Posttranslational Modification
197	Protein Variants
101	Purification (Commentary)
10	Reaction
44	Reaction Type
261	Reference
147	Source Tissue
79	Specific Activity [micromol/min/mg]
6	Storage Stability
533	Substrates and Products (Substrate)
40	Subunits
434	Synonyms
1	Systematic Name
55	Temperature Optimum [°C]
9	Temperature Range [°C]
21	Temperature Stability [°C]
127	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 4.2.99.18 - DNA-(apurinic or apyrimidinic site) lyase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.63 - 30000

12-mer oligodeoxyribonucleotide containing a natural AP site

2 entries

-

10000

12-mer oligodeoxyribonucleotide containing a tetrahydrofuran analogue at the natural AP site

Homo sapiens

-

wild-type, pH 7.5, 25°C

714206

-

7200 - 12000

18-mer containing P33-labeled tetrahydrofuran

2 entries

-

20555

30mer THF-T duplex

Helicobacter pylori

Q9ZJ98

pH 8.0, 37°C, recombinant His-tagged enzyme

749127

-

64.15 - 2620

5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3'

2 entries

-

0.63

12-mer oligodeoxyribonucleotide containing a natural AP site

Homo sapiens

-

mutant Y171F/P173L/N174K, pH 7.5, 25°C

714206

-

30000

12-mer oligodeoxyribonucleotide containing a natural AP site

Homo sapiens

-

wild-type, pH 7.5, 25°C

714206

-

7200

18-mer containing P33-labeled tetrahydrofuran

Cricetulus griseus

-

enzyme phosphorylated by casein kinase II, pH 7.5, 22°C

714966

-

12000

18-mer containing P33-labeled tetrahydrofuran

Cricetulus griseus

-

enzyme dephosphorylated by lambda phosphatase, pH 7.5, 22°C

714966

-

64.15

5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3'

Homo sapiens

-

recombinant enzyme

701682

-

2620

5'-Cy3-CAAGGTAGTrUATCCTTG-1-Black Hole Quencher1-3'

Rattus norvegicus

-

native enzyme

701682

-