4.2.3.95: (-)-alpha-cuprenene synthase
This is an abbreviated version!
For detailed information about (-)-alpha-cuprenene synthase, go to the full flat file.
Reaction
Synonyms
(-)-alpha-cuprenene synthase, Cop6
ECTree
4.2.3.95 (-)-alpha-cuprenene synthaseAdvanced search results
results (
results (Engineering
Engineering on EC 4.2.3.95 - (-)-alpha-cuprenene synthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C236A
-
the mutant produces 96% (-)-alpha-cuprenene, 3% gamma-cuprenene and 1% sesquiterpene products from (2E,6E)-farnesyl diphosphate (41.2% of wild type activity)
E237L
-
the mutant produces 96% (-)-alpha-cuprenene, 3% gamma-cuprenene and 1% unidentified sesquiterpene products from (2E,6E)-farnesyl diphosphate (59.4% of wild type activity)
N224D
-
the mutant makes a small amount of (6S)-beta-bisabolene (18%) from (2Z,6E)-farnesyl diphosphate, the mutation does not affect the product profile of Cop6 with its normal substrate (2E,6E)-farnesyl diphosphate
N240L
-
the mutant produces 98% (-)-alpha-cuprenene and 2% gamma-cuprenene from (2E,6E)-farnesyl diphosphate (31% of wild type activity)
additional information
-
in the Cop6 loop graft mutant Cop6L4, loop replacement has no influence on the selectivity of the enzyme for alpha-cuprenene, the mutation reduces the catalytic efficiency 3-17fold
