4.2.3.91: cubebol synthase

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For detailed information about cubebol synthase, go to the full flat file.

Reaction

Synonyms

Cop4

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.91 cubebol synthase

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Results	in table
1	AA Sequence
2	Cloned(Commentary)
2	KM Value [mM]
3	Metals/Ions
2	Organism
3	pH Optimum
1	pH Range
4	Protein Variants
1	Purification (Commentary)
1	Reaction
2	Reference
6	Substrates and Products (Substrate)
2	Synonyms
1	Systematic Name
2	Temperature Range [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 4.2.3.91 - cubebol synthase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

4L6

Coprinopsis cinerea

-

the mutant shows a 1000fold-decreased catalytic efficiency compared to the wild type enzyme

713839

H235P

Coprinopsis cinerea

-

the mutant makes (-)-germacrene D as the major cyclization product and also synthesizes beta-ylangene not synthesized by wild-type Cop4

713839

K233I

Coprinopsis cinerea

-

the mutation has only a moderate effect on product selectivity

713839

N239L

Coprinopsis cinerea

-

the mutant makes (-)-germacrene D as the major cyclization product and also synthesizes beta-ylangene not synthesized by wild-type Cop4

713839