4.2.3.9: aristolochene synthase
This is an abbreviated version!
For detailed information about aristolochene synthase, go to the full flat file.
Word Map on EC 4.2.3.9
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4.2.3.9
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cyclases
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phytoalexins
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roqueforti
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capsidiol
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sesquiterpenoids
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carbocation
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eudesmane
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trichodiene
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elicitor-treated
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+-delta-cadinene
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arboreum
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epi-isozizaene
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amorpha-4,11-diene
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sporotrichioides
- 4.2.3.9
- cyclases
-
phytoalexins
- roqueforti
- capsidiol
- sesquiterpenoids
-
carbocation
-
eudesmane
- trichodiene
-
elicitor-treated
-
+-delta-cadinene
- arboreum
- epi-isozizaene
- amorpha-4,11-diene
- sporotrichioides
Reaction
Synonyms
5-epi-aristolochene synthase, Ari1, aristolochene synthase, AS, ATAS, cyclase, farnesyl pyrophosphate, EAS, EC 2.5.1.40, EC 4.1.99.7, farnesylpyrophosphate cyclase, FPP-carbocyclase, sesquiterpene cyclase, synthase, aristolochene, TEAS
ECTree
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KM Value
KM Value on EC 4.2.3.9 - aristolochene synthase
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0.0143
(2Z,6E)-farnesyl diphosphate
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in 200 mM Tris-HCl (pH 7.5), 40 mM MgCl2, at 22°C
0.000042
mutant enzyme Q151H, at pH 8.0 and 30°C
0.000061
(2E,6E)-farnesyl diphosphate
mutant enzyme S303H, at pH 8.0 and 30°C
0.000099
(2E,6E)-farnesyl diphosphate
wild type enzyme, at pH 8.0 and 30°C
0.0001
(2E,6E)-farnesyl diphosphate
mutant enzyme Q151E, at pH 8.0 and 30°C
0.00012
(2E,6E)-farnesyl diphosphate
mutant enzyme S303A, at pH 8.0 and 30°C
0.00013
(2E,6E)-farnesyl diphosphate
-
mutant W334H, pH not specified in the publication, temperature not specified in the publication
0.00014
(2E,6E)-farnesyl diphosphate
mutant K251R, pH not specified in the publication, temperature not specified in the publication
0.00018
(2E,6E)-farnesyl diphosphate
mutant Y341F, pH not specified in the publication, temperature not specified in the publication
0.00024
(2E,6E)-farnesyl diphosphate
mutant K251Q, pH not specified in the publication, temperature not specified in the publication
0.00028
(2E,6E)-farnesyl diphosphate
-
mutant W334F, pH not specified in the publication, temperature not specified in the publication
0.00036
(2E,6E)-farnesyl diphosphate
mutant enzyme S303D, at pH 8.0 and 30°C
0.00041
(2E,6E)-farnesyl diphosphate
mutant enzyme N299A, at pH 8.0 and 30°C
0.00041
(2E,6E)-farnesyl diphosphate
mutant enzyme N299A/S303A, at pH 8.0 and 30°C
0.00053
(2E,6E)-farnesyl diphosphate
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.00053
(2E,6E)-farnesyl diphosphate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.00053
(2E,6E)-farnesyl diphosphate
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.00064
(2E,6E)-farnesyl diphosphate
mutant V88A, pH not specified in the publication, temperature not specified in the publication
0.0007
(2E,6E)-farnesyl diphosphate
mutant V88F, pH not specified in the publication, temperature not specified in the publication
0.00074
(2E,6E)-farnesyl diphosphate
mutant D203L, pH not specified in the publication, temperature not specified in the publication
0.00075
(2E,6E)-farnesyl diphosphate
-
mutant W334L, pH not specified in the publication, temperature not specified in the publication
0.001
(2E,6E)-farnesyl diphosphate
-
mutant W334Y, pH not specified in the publication, temperature not specified in the publication
0.00104
(2E,6E)-farnesyl diphosphate
mutant R200K, pH not specified in the publication, temperature not specified in the publication
0.00159
(2E,6E)-farnesyl diphosphate
mutant R340K, pH not specified in the publication, temperature not specified in the publication
0.00175
(2E,6E)-farnesyl diphosphate
mutant T89A, pH not specified in the publication, temperature not specified in the publication
0.0023
(2E,6E)-farnesyl diphosphate
wild-type, pH 7.5, temperature not specified in the publication
0.005
(2E,6E)-farnesyl diphosphate
mutant T89F, pH not specified in the publication, temperature not specified in the publication
0.0502
(2E,6E)-farnesyl diphosphate
mutant Y92C, pH 7.5, temperature not specified in the publication
0.0834
(2E,6E)-farnesyl diphosphate
mutant Y92A, pH 7.5, temperature not specified in the publication
0.7
(2E,6E)-farnesyl diphosphate
mutant L108A, pH not specified in the publication, temperature not specified in the publication
0.99
(2E,6E)-farnesyl diphosphate
mutant L108S, pH not specified in the publication, temperature not specified in the publication
1.76
(2E,6E)-farnesyl diphosphate
mutant L108F, pH not specified in the publication, temperature not specified in the publication
2.03
(2E,6E)-farnesyl diphosphate
mutant L108V, pH not specified in the publication, temperature not specified in the publication
0.0008
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assay under normal conditions and in D2O, 20 mM Tris, 5 mM MgCl2, 5 mM 2-mercaptoethanol and 15% glycerol
0.000012
pH 8.0, 30°C, mutant enzyme Y92F
0.0000148
trans,trans-farnesyl diphosphate
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pH 8.0, 30°C, recombinant enzyme
0.00015
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme E119Q
0.00032
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme E119D
0.00052
trans,trans-farnesyl diphosphate
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pH 8.0, 30°C, recombinant enzyme
0.0006
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, wild-type enzyme
0.0011
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme D116E
0.0014
trans,trans-farnesyl diphosphate
-
pH 8.0, 30°C, mutant enzyme E227D
0.0015
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme D116N
0.0023
trans,trans-farnesyl diphosphate
-
pH 7.5, 30°C, recombinant wild-type enzyme
0.0027
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme D115E
0.0033
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme N244D
0.0034
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme E252D
0.0039
trans,trans-farnesyl diphosphate
-
pH 8.0, 30°C, mutant enzyme N219D
0.0051
trans,trans-farnesyl diphosphate
-
wild-type enzyme, mutant enzyme F178Y
0.0055
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme S248A
0.0101
trans,trans-farnesyl diphosphate
pH 8.0, 30°C, mutant enzyme E252Q
0.0112
trans,trans-farnesyl diphosphate
-
wild-type enzyme, mutant enzyme F178V
0.05027
trans,trans-farnesyl diphosphate
pH 7.5, mutant enzyme Y92C
0.0834
trans,trans-farnesyl diphosphate
pH 7.5, mutant enzyme Y92A
0.1887
trans,trans-farnesyl diphosphate
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pH 7.5, 30°C, mutant enzyme Y92F