4.2.3.89: (+)-beta-caryophyllene synthase

This is an abbreviated version!
For detailed information about (+)-beta-caryophyllene synthase, go to the full flat file.

Reaction

Synonyms

(E)-beta-caryophyllene synthase, BCS, beta-caryophyllene synthase, CsSesquiTPS6a, CsSesquiTPS6b, CsSesquiTPS6c, DcTPS1, E-(beta)-caryophyllene synthase, GcoA, More, OkBCS, PlCAHS, PnCPS, PnTPS1, RtTPS1, RtTPS3, SQS6a, SQS6b, SQS7, TPS1, tps23, TPS3, VvGwECar2, WtDcTPS

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.3 Acting on phosphates

4.2.3.89 (+)-beta-caryophyllene synthase

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Results	in table
11	AA Sequence
1	Application
12	Cloned(Commentary)
1	Expression
19	General Information
2	kcat/KM [mM/s]
6	KM Value [mM]
3	Localization
7	Metals/Ions
1	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
17	Organism
1	Pathway
4	pH Optimum
1	pI Value
8	Protein Variants
5	Purification (Commentary)
2	Reaction
10	Reference
22	Source Tissue
18	Substrates and Products (Substrate)
5	Subunits
32	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
4	Turnover Number [1/s]

Purification

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Purification on EC 4.2.3.89 - (+)-beta-caryophyllene synthase

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Ni-NTA column chromatography

Streptomyces griseus

715629

recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Daucus carota subsp. carota

748003

recombinant C-terminally His6-tagged enzyme from Escherichia coli TOP10 cells by nickel affinity chromatography to homogeneity

Ocimum kilimandscharicum

A0A0E3KJK7

746974

recombinant His-tagged enzyme TPS1 from Escherichia coli strain Rosetta 2(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration

Piper nigrum

A0A1V0E492

746924

recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Rhodomyrtus tomentosa

A0A385J6X8, A0A385J6Y9

747883