4.2.3.87: alpha-guaiene synthase
This is an abbreviated version!
For detailed information about alpha-guaiene synthase, go to the full flat file.
Reaction
Synonyms
Pat177, patchoulol synthase, PTS, TPS, TPS24, VIT_19s0014g02590, VvGuaS, VvTPS24
ECTree
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Engineering
Engineering on EC 4.2.3.87 - alpha-guaiene synthase
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Y110S/V241E/P296S/H337Y
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the mutant produces 34.1% alpha-guaiene, 1.3% alpha-humulene and 64.6% delta-guaiene
Y110S/W144R/V241E/P296S/H337Y
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the mutant produces 31.1% alpha-guaiene, 1.5% alpha-humulene and 67.4% delta-guaiene
T414S
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme
T414S/V530M
site-directed mutagenesis, the mutant shows a guaiene synthase activity that is almost completely compromised, with only 5% of the volatile products comprising alpha-guaiene, and alpha-bulnesene not detectable. Instead, the major products are predominantly the 6,6 bicyclic sesquiterpenes selina-4,11-diene (40%), alpha-selinene (23%), epi-alpha-selinene (12%), and a hydroxylated selinene (9%) with the same reported RI as intermedeo
V530M
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme
additional information
the two polymorphisms within the active site of VvGuaS compared to VvPNSeInt (EC 4.2.3.86) correspond to T414S and V530M substitutions
additional information
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the two polymorphisms within the active site of VvGuaS compared to VvPNSeInt (EC 4.2.3.86) correspond to T414S and V530M substitutions