Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.3.87: alpha-guaiene synthase

This is an abbreviated version!
For detailed information about alpha-guaiene synthase, go to the full flat file.

Reaction

(2E,6E)-farnesyl diphosphate
=
alpha-guaiene
+
diphosphate

Synonyms

Pat177, patchoulol synthase, PTS, TPS, TPS24, VIT_19s0014g02590, VvGuaS, VvTPS24

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.87 alpha-guaiene synthase

Engineering

Engineering on EC 4.2.3.87 - alpha-guaiene synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
W144R/V241E/P296S/H337Y
-
inactive
Y110S/V241E/P296S/H337Y
-
the mutant produces 34.1% alpha-guaiene, 1.3% alpha-humulene and 64.6% delta-guaiene
Y110S/W144R/P296S/H337Y
-
inactive
Y110S/W144R/V241E/H337Y
-
inactive
Y110S/W144R/V241E/P296S
-
inactive
Y110S/W144R/V241E/P296S/H337Y
-
the mutant produces 31.1% alpha-guaiene, 1.5% alpha-humulene and 67.4% delta-guaiene
T414S
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme
T414S/V530M
site-directed mutagenesis, the mutant shows a guaiene synthase activity that is almost completely compromised, with only 5% of the volatile products comprising alpha-guaiene, and alpha-bulnesene not detectable. Instead, the major products are predominantly the 6,6 bicyclic sesquiterpenes selina-4,11-diene (40%), alpha-selinene (23%), epi-alpha-selinene (12%), and a hydroxylated selinene (9%) with the same reported RI as intermedeo
V530M
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme
additional information