4.2.3.75: (-)-germacrene D synthase

This is an abbreviated version!
For detailed information about (-)-germacrene D synthase, go to the full flat file.

Reaction

Synonyms

(-)-germacrene D synthase, Cop4, GDS, GerD, germacrene D synthase, Sc19, terpenoid cyclase, TPS, TPS07, TPS1, VitiM1610, ylangene/germacrene synthase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.75 (-)-germacrene D synthase

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Results	in table
34	AA Sequence
7	Cloned(Commentary)
1	Expression
2	kcat/KM [mM/s]
2	KM Value [mM]
5	Metals/Ions
3	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
57	Organism
2	Pathway
3	PDB ID
4	pH Optimum
1	pH Range
2	pI Value
2	Protein Variants
1	Purification (Commentary)
4	Reaction
10	Reference
13	Source Tissue
15	Substrates and Products (Substrate)
3	Subunits
14	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Turnover Number [1/s]

pH Optimum

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pH Optimum on EC 4.2.3.75 - (-)-germacrene D synthase

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10

Coprinopsis cinerea

-

at pH 10, the cyclization reaction ends with the hydride shift and deprotonation of the germacradienyl cation to yield (?)-germacrene D, 91% of total sesquiterpene products

714733

5

Coprinopsis cinerea

-

at pH 5.0 cyclization can proceed via a 1,6 ring closure to also yield a small amount of delta-cadinene, 12% of total sesquiterpene products, in addition to (?)-germacrene D

714733

7

Vitis vinifera

Q6Q3H3

assay at

663043

additional information

Coprinopsis cinerea

-

under both alkaline and acidic conditions Cop4 becomes a very selective enzyme with only one major product (?)-germacrene D compared to three major compounds produced under neutral reaction conditions. At pH 10, the cyclization reaction ends with the hydride shift and deprotonation of the germacradienyl cation to yield (?)-germacrene D, 91% of total sesquiterpene products, while at pH 5.0 cyclization can proceed via a 1,6 ring closure to also yield a small amount of delta-cadinene, 12% of total sesquiterpene products, in addition to (?)-germacrene D

714733