4.2.3.75: (-)-germacrene D synthase

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For detailed information about (-)-germacrene D synthase, go to the full flat file.

Reaction

Synonyms

(-)-germacrene D synthase, Cop4, GDS, GerD, germacrene D synthase, Sc19, terpenoid cyclase, TPS, TPS07, TPS1, VitiM1610, ylangene/germacrene synthase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.75 (-)-germacrene D synthase

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Results	in table
34	AA Sequence
7	Cloned(Commentary)
1	Expression
2	kcat/KM [mM/s]
2	KM Value [mM]
5	Metals/Ions
3	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
57	Organism
2	Pathway
3	PDB ID
4	pH Optimum
1	pH Range
2	pI Value
2	Protein Variants
1	Purification (Commentary)
4	Reaction
10	Reference
13	Source Tissue
15	Substrates and Products (Substrate)
3	Subunits
14	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Range [°C]
2	Turnover Number [1/s]

Temperature Range

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Temperature Range on EC 4.2.3.75 - (-)-germacrene D synthase

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

37

Coprinopsis cinerea

-

increasing the reaction temperature to 37°C decreases the fidelity of Cop4. At 37°C Cop4 generates a relative larger fraction of products beta-cubebene, sativene, delta-cadinene and beta-copaene that are derived from a cadinyl cation intermediate

714733

4 - 25

Coprinopsis cinerea

-

lowering the reaction temperature from 25°C to 4°C increases the selectivity of Cop4 for (?)-germacrene D and decreases the fraction of structurally unidentified sesquiterpene olefins by half

714733