4.2.3.56: gamma-humulene synthase
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For detailed information about gamma-humulene synthase, go to the full flat file.
Reaction
Synonyms
gamma-humulene synthase, gammaHS
ECTree
4.2.3.56 gamma-humulene synthaseAdvanced search results
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Engineering on EC 4.2.3.56 - gamma-humulene synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D343A
the mutation results in severely impaired activity for olefin production (estimated kcat reduction by more than 3000fold), the mutant produces 27.9% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 12.9% gamma-humulene (the main product of the wild type enzyme)
D343E
the mutant produces 34.4% sativene from (2E,6E)-farnesyl diphosphate as the main product and no gamma-humulene (the main product of the wild type enzyme), the mutant is capable of producing sesquiterpenols (61% nerolidol and 14% bisabolol, very similar to wild type distribution) at a rate comparable to that of olefin formation by the wild type enzyme
D488A
the mutant produces 40% sativene from (2E,6E)-farnesyl diphosphate as the main product and no gamma-humulene (the main product of the wild type enzyme)
D488E
the mutant produces 19.6% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 6.0% gamma-humulene (the main product of the wild type enzyme), olefin synthase activity is reduced to 3% of wild type enzyme
D488N
the mutant produces 21.7% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 18.7% gamma-humulene (the main product of the wild type enzyme)
D570N
the mutant produces 13.1% sativene from (2E,6E)-farnesyl diphosphate as the main product and 29.3% gamma-humulene (the main product of the wild type enzyme)
Y566F
the mutant produces 42.6% gamma-humulene (the main product of the wild type enzyme)
