4.2.3.52: (4S)-beta-phellandrene synthase (geranyl-diphosphate-cyclizing)

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For detailed information about (4S)-beta-phellandrene synthase (geranyl-diphosphate-cyclizing), go to the full flat file.

Word Map on EC 4.2.3.52

4.2.3.52

laryngectomy

laryngeal

oncological

endoscopic

arytenoid

five-year

surgeon

laryngological

epiglottis

non-surgical

grandis

oleoresin

intraoperatively

supraglottic

larynx

piriform

dysfunction-free

conifers

abies

limonene

cr

supracricoid

gastrostomy

Reaction

Synonyms

(-)-(4S)-beta-phellandrene synthase, (-)-beta-phellandrene synthase, beta-phellandrene synthase, OPHLS, phellandrene synthase, PHLS

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.3 Acting on phosphates

4.2.3.52 (4S)-beta-phellandrene synthase (geranyl-diphosphate-cyclizing)

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Results	in table
10	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
1	Inhibitors
1	kcat/KM [mM/s]
2	KM Value [mM]
1	Localization
7	Metals/Ions
2	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
10	Organism
1	Oxidation Stability
1	Pathway
2	pH Optimum
1	pH Range
1	pI Value
1	Purification (Commentary)
2	Reaction
10	Reference
5	Source Tissue
1	Storage Stability
15	Substrates and Products (Substrate)
2	Subunits
13	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Turnover Number [1/s]

Oxidation Stability

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Oxidation Stability on EC 4.2.3.52 - (4S)-beta-phellandrene synthase (geranyl-diphosphate-cyclizing)

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the enzyme is extremely sensitive to thiol oxidation. Cyclase activity is completely lost when the partially purified xylem preparation is dialyzed against buffer (pH 7.8) devoid of thiol-protecting reagents (dithiothreitol or 2-mercaptoethanol). Other reducing agents, such as sodium ascorbate (5 mM) or sodium metabisulfite (5 mM), fail to protect the cyclases from inactivation, whereas readdition of 5 mM dithiothreitol to the dialyzed preparations restores 54% of the original activity

Pinus contorta

704380