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4.2.3.37: epi-isozizaene synthase

This is an abbreviated version!
For detailed information about epi-isozizaene synthase, go to the full flat file.

Word Map on EC 4.2.3.37

Reaction

(2E,6E)-farnesyl diphosphate
=
(+)-epi-isozizaene
 +
diphosphate

Synonyms

EIZS, epi-isozizaene synthase, SAV_3032, SCO5222 protein, TPS2

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.37 epi-isozizaene synthase

Crystallization

Crystallization on EC 4.2.3.37 - epi-isozizaene synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified wild-type enzyme and mutant F95N in complex with Mg2+, benzyl triethylammonium cation (BTAC), and diphosphate, sitting drop vapor diffusion method, mixing of 5 mm nl of 5 mg/ml protein in 300 mM NaCl, 20 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 2 mM tris(2-carboxyethyl)phosphine (TCEP), 10% glycerol, 2 mM sodium pyrophosphate, and 2 mM BTAC with 600 nl of precipitant solution containing 0.17 M sodium acetate trihydrate, 85 mM sodium cacodylate trihydrate, pH 6.5, 25.5% PEG 8000, and 15% glycerol, addition of 100 nL of the 100fold dilution of wild-type enzyme crystallization seed stock, equilibration against a 0.1 ml reservoir of the precipitant solution at room temperature, 6 days, X-ray diffraction structure determination and analysis at 1.8 A and 2.51 A resolution, respectively
sitting drop vapor diffusion method