4.2.3.32: levopimaradiene synthase
This is an abbreviated version!
For detailed information about levopimaradiene synthase, go to the full flat file.
Word Map on EC 4.2.3.32
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Synonyms
abietadiene synthase, levopimaradiene/abietadiene synthase, LP synthase, LPS, More, PaLAS, TPS-LAS
ECTree
4.2.3.32 levopimaradiene synthaseAdvanced search results
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Engineering on EC 4.2.3.32 - levopimaradiene synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A620T
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130% of wild-type productivity. Products are 87% levopimaradiene, 11% abieta-7,13-diene, and 2% sandaracopimaradiene
C618N
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20% of wild-type productivity. Products are 91% levopimaradiene, 9% abieta-7,13-diene, and traces of sandaracopimaradiene
G854T
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140% of wild-type productivity. Products are 79% levopimaradiene, 14% abieta-7,13-diene, and 7% sandaracopimaradiene
I855L
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70% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene
K723S
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180% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene
L619F
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150% of wild-type productivity. Products are 94% levopimaradiene, 6% abieta-7,13-diene, and traces of sandaracopimaradiene
L696Q
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160% of wild-type productivity. Products are 86% levopimaradiene, 7% abieta-7,13-diene, and 7% sandaracopimaradiene
M593I
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370% of wild-type productivity. Products are 83% levopimaradiene, 12% abieta-7,13-diene, and 5% sandaracopimaradiene
M593I/Y700F
the mutant shows 23fold increased activity compared to the wild type enzyme
N838E
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220% of wild-type productivity. Products are 92% levopimaradiene, 6% abieta-7,13-diene, and 2% sandaracopimaradiene
Y700F
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500% of wild-type productivity. Products are 80% levopimaradiene, 15% abieta-7,13-diene, and 5% sandaracopimaradiene
Y700H
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40% of wild-type productivity. Products are 71% levopimaradiene, traces of abieta-7,13-diene, and 29% sandaracopimaradiene
Y700M
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500% of wild-type productivity. Products are 80% levopimaradiene, 13% abieta-7,13-diene, and 7% sandaracopimaradiene
Y700W
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400% of wild-type productivity. Products are 85% levopimaradiene, 7% abieta-7,13-diene, and 8% sandaracopimaradiene
W679L/Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
Y686H/A713S A713S
main products are isopimaradiene and sandaracopimaradiene
additional information
additional information
removal of 60 or 79 N-terminal residues increases levopimaradiene production, deletion of 128 N-terminal residues results in loss of catalytic activity
additional information
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removal of 60 or 79 N-terminal residues increases levopimaradiene production, deletion of 128 N-terminal residues results in loss of catalytic activity
additional information
swapping of residues 568-640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes
additional information
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swapping of residues 568-640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes
additional information
using a combination of protein and genetic engineering, significant improvements in the production of sclareol and several other isoprenoids is achieved, including cis-abienol, abietadiene, and beta-carotene. Recombinant expression of enzyme PaLAS in Saccharomyces cerevisiae AM94 cells, coexpression with different Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS
