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4.2.3.32: levopimaradiene synthase

This is an abbreviated version!
For detailed information about levopimaradiene synthase, go to the full flat file.

Word Map on EC 4.2.3.32

Reaction

(+)-copalyl diphosphate
=
abieta-8(14),12-diene
+
diphosphate

Synonyms

abietadiene synthase, levopimaradiene/abietadiene synthase, LP synthase, LPS, More, PaLAS, TPS-LAS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.32 levopimaradiene synthase

Cloned

Cloned on EC 4.2.3.32 - levopimaradiene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain w303-1a
expression in Arabidopsis thaliana
expression in Escherichia coli
expression of green fluorescent fusion protein in tobacco cells
gene TPS-LAS, recombinant expression in Saccharomyces cerevisiae strain AM94, a yeast strain engineered for the production of isoprenoids, which contains three chromosomally integrated copies of a degradation stabilized variant of HMG2 and a heterozygous deletion of ERG9, and in strain AM204, each with or without coexpression of yeast protein Erg20p(F96C), a yeast farnesyl diphosphate synthase, overview. Coexpression with different Saccharomyces cerevisiae ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS