4.2.3.30: ent-pimara-8(14),15-diene synthase
This is an abbreviated version!
For detailed information about ent-pimara-8(14),15-diene synthase, go to the full flat file.
Reaction
Synonyms
AN1594, ANIA_01594, ent-kaurene synthase-like 5, ent-pimara-8(14),15-diene synthase, kaurene synthase-like enzyme 5, KS-like 5j, KSL5, OrKSL5, OsKS5, OsKSL5j, pbcA, PD synthase
ECTree
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Substrates Products
Substrates Products on EC 4.2.3.30 - ent-pimara-8(14),15-diene synthase
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REACTION DIAGRAM
ent-pimara-8(14),15-diene + diphosphate
Q5BCY6
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-
-
?
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
Q5BCY6
-
-
-
?
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139
Q5BCY6
-
-
-
?
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
-
activity of the mutant I645T of kaurene synthase, EC 4.2.3.19
-
-
?
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
LC169117
-
-
-
?
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
-
-
-
?
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
-
activity of the mutant I664T of kaurene synthase, EC 4.2.3.19
-
-
?
ent-copalyl diphosphate
ent-pimara-8(14),15-diene + diphosphate
-
activity of the mutant I741T of kaurene synthase, EC 4.2.3.19
-
-
?
?
-
Q5BCY6
GC/MS analysis of enzyme products
-
-
?
additional information
?
-
Q5BCY6
GC/MS analysis of enzyme products
-
-
?
additional information
?
-
Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139
Q5BCY6
GC/MS analysis of enzyme products
-
-
?
additional information
?
-
-
the mutant MpKS:I645T produces almost entirely ent-pimara-8(14),15-diene
-
-
?
additional information
?
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GC-MS reaction product analysis. The distinct function of two alleles of a KSL from rice, OsKSL5i and OsKSLj, identifies a single residue switch with a profound effect on not only OsKSL5 product outcome but also that of land plant KSs more broadly, specifically, replacement of a key isoleucine with threonine, which interrupts formation of the tetracyclic ent-isokaurene at the tricyclic stage, leading to production of ent-pimaradiene instead
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-
?
additional information
?
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the OsKSL5 mutant I664A produces almost exclusively ent-pimara-8(14),15-diene, it also produces low amounts of ent-copalol, the dephosphorylated derivative of ent-copalyl diphosphate
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-
?
additional information
?
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the mutant PpCPS/KS:I741T produces almost entirely ent-pimara-8(14),15-diene
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-
?