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4.2.3.3: methylglyoxal synthase

This is an abbreviated version!
For detailed information about methylglyoxal synthase, go to the full flat file.

Word Map on EC 4.2.3.3

Reaction

glycerone phosphate
=
2-oxopropanal
+
phosphate

Synonyms

EC 4.2.99.11, methylglyoxal synthase, methylglyoxal synthetase, MGS, MgsA, msgA, synthase, methylglyoxal

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.3 methylglyoxal synthase

Temperature Stability

Temperature Stability on EC 4.2.3.3 - methylglyoxal synthase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 75
100% activity after 15 min
100
-
1 min, complete inactivation
70 - 80
the enzyme shows no loss of activity after 150 min at 70°C, shows about 70% activity after 150 min at 75°C, and is almost inactivated after 60 min at 80°C
80
wild-type, half-life 3 min, mutant with C-terminal extension YQRYLADRLK, half-life 10 min
85
10 min, wild-type retains 84 of initial activity, mutant R97E shows complete loss of activity
additional information
-
phosphate stabilizes towards both cold-induced inactivation and against heat-induced inactivation