Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.3.25: S-linalool synthase

This is an abbreviated version!
For detailed information about S-linalool synthase, go to the full flat file.

Word Map on EC 4.2.3.25

Reaction

geranyl diphosphate
+
H2O
=
(3S)-linalool
 +
diphosphate

Synonyms

(+)-(3S)-linalool nerolidol synthase, (-)-linalool synthase, (S)-linalool synthase, bLINS, FaNES, LIN-NERS1, LIN-NERS2, linalool synthase, linalool/nerolidol synthase, LIS, LIS1, LIS2, LS1, More, S-linalool synthase, S-LIS, Tps-5073L4, TPS12, TPS14, TPS4

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.25 S-linalool synthase

Cloned

Cloned on EC 4.2.3.25 - S-linalool synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA from strawberry with plastid targeting and a synthetic intron is placed under the control of the wound inducible proteinase inhibitor 2 (PI2) promoter from potato. The construct is transformed to Arabidopsis thaliana ecotype Columbia 0
Fragaria sp.
-
cloning in a binary vector for introduction into Agrobacterium tumefaciens strain LBA4404
-
Escherichia coli strain DH5alpha is used as the strain for cloning experiments and plasmid amplification. Saccharomyces cerevisiae wine strain T73-4 is used for functional expression of plasmids bearing the expression cassette.
expressed in Escherichia coli ArcticExpress (DE3) cells
-
expressed in Saccharomyces cerevisiae
-
functional expression in transgenic Petunia hybrida plants
-
gene LIS2, partial genomic library construction, DNA sequence determination and analysis, promoter sequence analysis, gene structure
His-tagged version expressed in Escherichia coli
into the pNZ8150 vector for expression in Lactococcus lactis
-
isoform TPS12 is expressed in Nicotiana tabacum
-
partial genomic library construction, DNA sequence determination and analysis, gene structure
-
partial genomic library construction, DNA sequence determination and analysis, promoter sequence analysis, gene structure
-
partial protein sequencing and cDNA library construction, DNA and amino acid determination and analysis
transient expression in Nicotiana benthamiana, His-tagged version expressed in Escherichia coli