4.2.3.18: abieta-7,13-diene synthase
This is an abbreviated version!
For detailed information about abieta-7,13-diene synthase, go to the full flat file.
Reaction
Synonyms
(-)-abietadiene synthase, abietadiene cyclase, abietadiene synthase, abietadiene/levopimaradiene synthase, AGAS, cyclase, abietadiene, PaLAS, PdABS, PtTPS-LAS, TPS-LAS
ECTree
4.2.3.18 abieta-7,13-diene synthaseAdvanced search results
results (
results (Engineering
Engineering on EC 4.2.3.18 - abieta-7,13-diene synthase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A723S
product is switched from abietadienes to 75% isopimara-7,15-diene + 21% sandaracopimaradiene
D404A
D621A
D625A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
D766A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
D845A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E589A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E699A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E773A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
E778A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
H348A/D621A
the mutant also produces 8alpha-hydroxy-copalyl diphosphate from geranylgeranyl diphosphate
N451A
this mutation reduces class II (protonation-initiated cyclization) activity about 100fold, with essentially no effect on class I (ionization-initiated cyclization) activity
N765A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R584A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R586A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
R762A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
S721A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
T617A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
T769A
T848A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
Y841F
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
A620T
-
130% of wild-type productivity. Products are 87% levopimaradiene, 11% abieta-7,13-diene, and 2% sandaracopimaradiene
C618N
-
20% of wild-type productivity. Products are 91% levopimaradiene, 9% abieta-7,13-diene, and traces of sandaracopimaradiene
G854T
-
140% of wild-type productivity. Products are 79% levopimaradiene, 14% abieta-7,13-diene, and 7% sandaracopimaradiene
I855L
-
70% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene
K723S
-
180% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene
L619F
-
150% of wild-type productivity. Products are 94% levopimaradiene, 6% abieta-7,13-diene, and traces of sandaracopimaradiene
L696Q
-
160% of wild-type productivity. Products are 86% levopimaradiene, 7% abieta-7,13-diene, and 7% sandaracopimaradiene
M593I
-
370% of wild-type productivity. Products are 83% levopimaradiene, 12% abieta-7,13-diene, and 5% sandaracopimaradiene
N838E
-
220% of wild-type productivity. Products are 92% levopimaradiene, 6% abieta-7,13-diene, and 2% sandaracopimaradiene
Y700F
-
500% of wild-type productivity. Products are 80% levopimaradiene, 15% abieta-7,13-diene, and 5% sandaracopimaradiene
Y700H
-
40% of wild-type productivity. Products are 71% levopimaradiene, traces of abieta-7,13-diene, and 29% sandaracopimaradiene
Y700M
-
500% of wild-type productivity. Products are 80% levopimaradiene, 13% abieta-7,13-diene, and 7% sandaracopimaradiene
Y700W
-
400% of wild-type productivity. Products are 85% levopimaradiene, 7% abieta-7,13-diene, and 8% sandaracopimaradiene
W679L/Y686H/A713S/V717L
products are isopimaradiene and sandaracopimaradiene
Y686H/A713S A713S
main products are isopimaradiene and sandaracopimaradiene
additional information
swapping of residues 568640 of isopimaradiene synhase to corresponding residues 560-632 of levopimaradiene/abietadiene synthase results in complete reversion of the product profiles of the two enzymes
D621A
this substitution eliminates the subsequently acting class I activity
T769A
-
no effect on geranylgeranyl diphosphate reaction, but lower turnover with copalyl diphosphate than wild-type
