4.2.3.17: taxadiene synthase
This is an abbreviated version!
For detailed information about taxadiene synthase, go to the full flat file.
Word Map on EC 4.2.3.17
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4.2.3.17
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taxus
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paclitaxel
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taxane
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brevifolia
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tx
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diterpene
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diterpenoids
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taxoids
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carbocation
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medicine
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10-deacetylbaccatin
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baccata
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taxol-producing
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cuspidata
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mairei
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ggpps
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synthesis
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drug development
- 4.2.3.17
- taxus
- paclitaxel
-
taxane
- brevifolia
- tx
-
diterpene
-
diterpenoids
-
taxoids
-
carbocation
- medicine
-
10-deacetylbaccatin
- baccata
-
taxol-producing
- cuspidata
- mairei
- ggpps
- synthesis
- drug development
Reaction
Synonyms
geranylgeranyl pyrophosphate cyclase, taxa-4(5),11(12)-diene synthase, taxadiene synthase, taxadiene synthase 5, TXS
ECTree
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Specific Activity
Specific Activity on EC 4.2.3.17 - taxadiene synthase
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additional information
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a relatively high level of activity is observed at day 4, when it is 2.8-, 6.8-, 3.2- and 1.7fold higher, respectively, in the cultures treated with 25, 50, 100 and 200 mg/l taxol than in the untreated control (6.64 pkat/mg of protein). Enzyme activity then decreases markedly from days 4 to 12 and thereafter is measurable only at a very low level until day 20.
additional information
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Replacement of the geranylgeranyl diphosphate synthase with the enzyme from Sulfolobus acidocaldarius and codon optimizing of the Taxus chinensis taxadiene synthase gene to ensure high-level expression, resulting in a 40fold increase in taxadiene to 8.770.85 mg/l as well as significant amounts of geranylgeraniol (33.1 mg/l)