4.2.3.15: myrcene synthase
This is an abbreviated version!
For detailed information about myrcene synthase, go to the full flat file.
Reaction
Synonyms
AaTPS2, AcTPS2, AtTPS10, EC 4.1.99.9, myrcene synthase, myrcene/(E)-beta-ocimene synthase, TPS10, TPS2
ECTree
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Engineering
Engineering on EC 4.2.3.15 - myrcene synthase
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K514A
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AcTPS2 mutant, 90% reduction in monoterpene synthase activity compared to wild-type enzyme
K514S
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AcTPS2 mutant, presence of Ser is not able to rescue any of the activity lost after the removal of the Lys residue
additional information
the N-terminal signal peptide of AaTPS2 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3)
additional information
-
the N-terminal signal peptide of AaTPS2 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3)