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4.2.3.146: cyclooctat-9-en-7-ol synthase

This is an abbreviated version!
For detailed information about cyclooctat-9-en-7-ol synthase, go to the full flat file.

Word Map on EC 4.2.3.146

Reaction

geranylgeranyl diphosphate
+
H2O
=
cyclooctat-9-en-7-ol
 +
diphosphate

Synonyms

cetB2, CotB2, CYC, diterpene cyclooctatin synthase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.146 cyclooctat-9-en-7-ol synthase

Cloned

Cloned on EC 4.2.3.146 - cyclooctat-9-en-7-ol synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
gene cotB2, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene cotB2, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), selenomethionine-substituted CotB2 was expressed in Escherichia coli strain B834(DE3)
overexpression of the cyclooctatin biosynthetic gene cluster in Escherichia coli