4.2.3.13: (+)-delta-cadinene synthase

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For detailed information about (+)-delta-cadinene synthase, go to the full flat file.

Reaction

Synonyms

(+)-delta-cadinene synthase, (+)-delta-cadinene synthase isoenzyme XC1, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-alpha-cadinene-forming), bifunctional sesquiterpene synthase 1, CAD, cadinene synthase, cadinol/cadinene synthase, CDN1, Cdn1-C1, Cdn1-C14, Cop4, cyclase, delta-cadinene, DCS, Dcs1, delta-cadinene cyclase, delta-cadinene synthase, EC 4.6.1.11, farnesyl diphosphate-delta-cadinene cyclase, PnCO/CDS, PnTPS2, sesquiterpene synthase, TPS1, TPS2

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.13 (+)-delta-cadinene synthase

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Results	in table
1182	AA Sequence
2	Application
1	CAS Registry Number
17	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
10	General Information
1	General Stability
1	IC50 Value
1	Inhibitors
19	kcat/KM [mM/s]
30	KM Value [mM]
7	Metals/Ions
8	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
45	Organism
5	Pathway
4	PDB ID
4	pH Optimum
2	pH Range
28	Protein Variants
12	Purification (Commentary)
4	Reaction
2	Reaction Type
28	Reference
30	Source Tissue
16	Specific Activity [micromol/min/mg]
2	Storage Stability
37	Substrates and Products (Substrate)
5	Subunits
34	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
25	Turnover Number [1/s]

Purification

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Purification on EC 4.2.3.13 - (+)-delta-cadinene synthase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

affinity chromatography, chromatography on glutathione-Sepharose-4B column and anion-exchange column

Gossypium arboreum

Q39761

648584

CAD1-A isoenzyme

Gossypium arboreum

-

648579

DEAE-Sepharose column chromatography

Gossypium arboreum

Q39761

748810

N-terminal fusion of 10x His-tag, purification by affinity chromatography under native and denaturing conditions

Gossypium hirsutum

-

666642

Ni-NTA affinity chromatography

Helianthus annuus

Q4U3F6

702923

Ni-NTA affinity column chromatography

Helianthus annuus

Q4U3F6

702923

Ni-NTA column chromatography

Gossypium arboreum

Q39761

747551

purification of a recombinant enzyme using histidine affinity chromatography

Gossypium arboreum

Q39760, Q39761

648582

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, diaylsis, and ultrafiltration

Gossypium arboreum

Q39761

747551

slurry homogenization and centrifugation

Gossypium hirsutum

-

648583

soluble at pH 11.6, ion exchange chromatography (DEAE), gel filtration

Gossypium arboreum

Q39761

702321

to homogeneity, several chromatographic steps, including hydroxyapatite, phenyl-agarose and anion-exchange chromatography

Gossypium hirsutum

-

648578