4.2.3.13: (+)-delta-cadinene synthase

This is an abbreviated version!
For detailed information about (+)-delta-cadinene synthase, go to the full flat file.

Reaction

Synonyms

(+)-delta-cadinene synthase, (+)-delta-cadinene synthase isoenzyme XC1, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-alpha-cadinene-forming), bifunctional sesquiterpene synthase 1, CAD, cadinene synthase, cadinol/cadinene synthase, CDN1, Cdn1-C1, Cdn1-C14, Cop4, cyclase, delta-cadinene, DCS, Dcs1, delta-cadinene cyclase, delta-cadinene synthase, EC 4.6.1.11, farnesyl diphosphate-delta-cadinene cyclase, PnCO/CDS, PnTPS2, sesquiterpene synthase, TPS1, TPS2

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.13 (+)-delta-cadinene synthase

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Results	in table
1182	AA Sequence
2	Application
1	CAS Registry Number
17	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
10	General Information
1	General Stability
1	IC50 Value
1	Inhibitors
19	kcat/KM [mM/s]
30	KM Value [mM]
7	Metals/Ions
8	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
45	Organism
5	Pathway
4	PDB ID
4	pH Optimum
2	pH Range
28	Protein Variants
12	Purification (Commentary)
4	Reaction
2	Reaction Type
28	Reference
30	Source Tissue
16	Specific Activity [micromol/min/mg]
2	Storage Stability
37	Substrates and Products (Substrate)
5	Subunits
34	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
25	Turnover Number [1/s]

KM Value

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KM Value on EC 4.2.3.13 - (+)-delta-cadinene synthase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.00058 - 0.0184

(2E,6E)-farnesyl diphosphate

20 entries

0.0024 - 0.043

(2E,6Z)-farnesyl diphosphate

4 entries

0.0017 - 0.0106

2-trans,6-trans-farnesyl diphosphate

4 entries

0.00065

nerolidyl diphosphate

Gossypium arboreum

Q39761

pH 7.5, 30ºC, recombinant CDN1-C1 enzyme

648584

additional information

additional information

Gossypium arboreum

Q39761

Michaelis-Menten steady-state kinetics, overview

747551

-

0.00058

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

wild type enzyme, at pH 7.5 and 25°C

747551

0.00058

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant His6-tagged wild-type enzyme, pH 7.5, 25°C

747551

0.00145

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

mutant enzyme W279E, at pH 7.5 and 25°C

747551

0.00145

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant mutant W279E, pH 7.5, 25°C

747551

0.0019

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

mutant enzyme W279M, at pH 7.5 and 25°C

747551

0.0019

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant mutant W279M, pH 7.5, 25°C

747551

0.0022

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant mutant W279A, pH 7.5, 25°C

747551

0.00223

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

mutant enzyme W279A, at pH 7.5 and 25°C

747551

0.0032

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

wild type enzyme, at pH 8.0 and 30°C

748810

0.0032

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

wild type enzyme, at pH 7.5 and 25°C

747551

0.00428

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

mutant enzyme W279Y, at pH 7.5 and 25°C

747551

0.0043

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant mutant W279Y, pH 7.5, 25°C

747551

0.0048

(2E,6E)-farnesyl diphosphate

Phyla dulcis

J7LP58

pH 7.5, 30°C

718701

0.008

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

mutant enzyme W279Q, at pH 7.5 and 25°C

747551

0.008

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant mutant W279Q, pH 7.5, 25°C

747551

0.0092

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant mutant W279D, pH 7.5, 25°C

747551

0.00924

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

mutant enzyme W279D, at pH 7.5 and 25°C

747551

0.00945

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

mutant enzyme W279L, at pH 7.5 and 25°C

747551

0.0095

(2E,6E)-farnesyl diphosphate

Gossypium arboreum

Q39761

recombinant mutant W279L, pH 7.5, 25°C

747551

0.0184

(2E,6E)-farnesyl diphosphate

Piper nigrum

A0A1V0E4A6

pH and temperature not specified in the publication

746924

0.0024

(2E,6Z)-farnesyl diphosphate

Gossypium arboreum

Q39761

D451A mutant protein

702321

0.0031

(2E,6Z)-farnesyl diphosphate

Gossypium arboreum

Q39761

D452A mutant protein

702321

0.0032

(2E,6Z)-farnesyl diphosphate

Gossypium arboreum

Q39761

wild-type protein

702321

0.043

(2E,6Z)-farnesyl diphosphate

Gossypium arboreum

Q39761

D308A mutant protein

702321

0.0017

2-trans,6-trans-farnesyl diphosphate

Gossypium arboreum

Q39760, Q39761

pH 7, 30°C, fusion protein composed of the pXC1-encoded protein and the histidine leader peptide derived from pET28

648582

0.00605

2-trans,6-trans-farnesyl diphosphate

Gossypium arboreum

Q39761

pH 7.5, 30ºC, recombinant CDN1-C1 enzyme

648584

0.007

2-trans,6-trans-farnesyl diphosphate

Gossypium arboreum

-

isoenzyme CAD1-A, pH 8.7, 30°C

648579

0.0106

2-trans,6-trans-farnesyl diphosphate

Gossypium hirsutum

-

-

666642