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4.2.3.13: (+)-delta-cadinene synthase

This is an abbreviated version!
For detailed information about (+)-delta-cadinene synthase, go to the full flat file.

Word Map on EC 4.2.3.13

Reaction

(2E,6E)-farnesyl diphosphate
=
(+)-delta-Cadinene
 +
diphosphate

Synonyms

(+)-delta-cadinene synthase, (+)-delta-cadinene synthase isoenzyme XC1, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, (+)-alpha-cadinene-forming), bifunctional sesquiterpene synthase 1, CAD, cadinene synthase, cadinol/cadinene synthase, CDN1, Cdn1-C1, Cdn1-C14, Cop4, cyclase, delta-cadinene, DCS, Dcs1, delta-cadinene cyclase, delta-cadinene synthase, EC 4.6.1.11, farnesyl diphosphate-delta-cadinene cyclase, PnCO/CDS, PnTPS2, sesquiterpene synthase, TPS1, TPS2

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.13 (+)-delta-cadinene synthase

Cloned

Cloned on EC 4.2.3.13 - (+)-delta-cadinene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Saccharomyces cerevisiae strain EPY300 as a thioredoxin-fusion protein
expression in Escherichia coli
expression in Escherichia coli of a cDNA isolated and amplified from a cell culture infected with Verticillium dahliae
expression in Escherichia coli of CDN1-C1
expression in Escherichia coli of the CAD1-A isoenzyme, 80% of homology with CAD1-C isoenzyme
-
expression in Saccharomyces cerevisiae and Escherichia coli
exprsssion in Escherichia coli and Saccharomyces cerevisiae
gene Cdn1-C1, real-time PCR expression analysis
gene PnTPS2, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain EPY300. PnTPS2-transformed yeast cells accumulate delta-cadinol as a major product (91%) and smaller amounts of alpha-cadinene (2%) and delta-cadinene (7%)
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
thioredoxin-fusion protein expressed in Escherichia coli, expressed in Saccharomyces cerevisiae EPY300