4.2.3.129: (+)-sativene synthase

This is an abbreviated version!
For detailed information about (+)-sativene synthase, go to the full flat file.

Reaction

Synonyms

Cop4, sesquiterpene synthase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.129 (+)-sativene synthase

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Results	in table
1	Activating Compound
1	AA Sequence
3	Cloned(Commentary)
7	General Information
4	KM Value [mM]
4	Metals/Ions
5	Natural Substrates/ Products (Substrates)
5	Organism
1	pH Optimum
1	pH Range
7	Protein Variants
1	Reaction
5	Reference
1	Source Tissue
8	Substrates and Products (Substrate)
2	Subunits
7	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Metals Ions

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Metals Ions on EC 4.2.3.129 - (+)-sativene synthase

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mg2+

2 entries

Mn2+

Coprinopsis cinerea

A8NU13

substitution of Mg2+ with Mn2+ as the divalent metal ion shifts the product profile of Cop4 to germacrene D, disfavoring subsequent ring closures that produce the cadinyl cation and its tricyclic descendents

714733

NaCl

Coprinopsis cinerea

A8NU13

does not affect the product specificity of Cop4 significantly at 1 M

714733

Mg2+

Coprinopsis cinerea

A8NU13

required

705783

Mg2+

Coprinopsis cinerea

A8NU13

required, substitution of Mg2+ with Mn2+ as the divalent metal ion shifts the product profile of Cop4 to germacrene D, disfavoring subsequent ring closures that produce the cadinyl cation and its tricyclic descendents. Two consensus sequences - an aspartate rich DDXXD/E and a NSE/DTE motif - located at the entrance of the active site coordinate a trinuclear Mg2+ cluster that ligands the diphosphate moiety of the isoprenoid substrate, positions the isoprenyl chain in the binding pocket and triggers closure of the active site along with diphosphate cleavage to generate an initial transoid, allylic carbocation

714733