4.2.3.128: beta-cubebene synthase

This is an abbreviated version!
For detailed information about beta-cubebene synthase, go to the full flat file.

Reaction

Synonyms

(Z)-beta-cubebene/alpha-copaene synthase, caryophyllene/cubebene synthase, Cop4, CoTPS2, CsSesquiTPS1, Mg25, sesquiterpene synthase, TPS2, VvShirazTPS-Y2, VvTPS

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.3 Acting on phosphates

4.2.3.128 beta-cubebene synthase

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Results	in table
1	Activating Compound
5	AA Sequence
6	Cloned(Commentary)
12	General Information
4	KM Value [mM]
1	Localization
10	Metals/Ions
13	Natural Substrates/ Products (Substrates)
8	Organism
1	Pathway
3	pH Optimum
4	pH Range
6	Protein Variants
1	Purification (Commentary)
3	Reaction
8	Reference
13	Source Tissue
21	Substrates and Products (Substrate)
3	Subunits
13	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

pH Range

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pH Range on EC 4.2.3.128 - beta-cubebene synthase

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5 - 10

mutant enzyme K233I

6.5 - 8.5

no activity below or above

720729

Coprinopsis cinerea

A8NU13

wild-type enzyme and mutant H235P

713839

additional information

Coprinopsis cinerea

A8NU13

changing the pH of the reaction drastically alters the fidelity of Cop4 and makes it a highly selective enzyme. Increasing the reaction temperature to 37°C decreases the fidelity of Cop4. At this temperature Cop4 generates a relative larger fraction of products beta-cubebene, sativene, delta-cadinene and beta-copaene, that are derived from a cadinyl cation intermediate. The histidine side chain in the Cop4 loop, in particular, has a strong impact on the net charge of the loop at different pH values

714733