4.2.3.128: beta-cubebene synthase
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For detailed information about beta-cubebene synthase, go to the full flat file.
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Synonyms
(Z)-beta-cubebene/alpha-copaene synthase, caryophyllene/cubebene synthase, Cop4, CoTPS2, CsSesquiTPS1, Mg25, sesquiterpene synthase, TPS2, VvShirazTPS-Y2, VvTPS
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Cloned
Cloned on EC 4.2.3.128 - beta-cubebene synthase
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expression of wild-type and mutant enzymes in Escherichia coli strain JM109
gene cop4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain JM109
gene CsSesquiTPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, the enzyme encoded by CsSesquiTPS1 produces a 62:38% mixture of (Z)-beta-cubebene and alpha-copaene
gene Mg25, DNA and amino acid sequence determination and analysis, Mg25 has one single intron located near the 5' terminus of the gene, expression analysis and phylogenetic analysis
gene TPS2, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, transient and functional recombinant expression of YFP-tagged enzyme CoTPS2 in transgenic Nicotiana benthamiana leaves in cytosol under control of CaMV 35S promoter via Agrobacterium tumefaciens-mediated transfection method
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