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4.2.3.12: 6-pyruvoyltetrahydropterin synthase

This is an abbreviated version!
For detailed information about 6-pyruvoyltetrahydropterin synthase, go to the full flat file.

Word Map on EC 4.2.3.12

Reaction

7,8-dihydroneopterin 3'-triphosphate
=
6-pyruvoyl-5,6,7,8-tetrahydropterin
 +
Triphosphate

Synonyms

2-amino-4-oxo-6-(erythro-1',2',3'-trihydroxypropyl)-7,8-dihydroxypterdine triphosphate lyase, 6-(1,2-dioxopropyl)tetrahydropterin synthase, 6-carboxy-5,6,7,8-tetrahydropterin synthase, 6-pyruvoyl tetrahydrobiopterin synthase, 6-pyruvoyl-tetrahydropterin synthase, 6-pyruvoyltetrahydropterin synthase, 6-pyruvoyltetrahydropterin synthase paralog, 6-pyruvoyltetrahydropterin synthase [16-cysteine] (human clone lamda HSY2 gene PCBD subunit), 6-pyruvoyltetrahydropterin synthase [25-glutamine] (human clone lambdaHSY2 gene PCBD subunit), 6-pyruvoyltetrahydropterin synthase [de-57-valine] (human clone lambdaHSY2 gene PCBD subunit), 6-pyruvoyltetrahydropterin synthase-like protein, EC 4.6.1.10, ePTPS, PH0634, PPH4 synthase, PPH4S, protein purple, PTP synthase, PTPS, PTPS homologue, PTPS-III, PTS, pyruvoyltetrahydropterin synthase, SCO 6650, synthase, 6-pyruvoyltetrahydropterin, synthase, 6-pyruvoyltetrahydropterin [16-cysteine] (human clone lamda HSY2 gene PCBD subunit), synthase, 6-pyruvoyltetrahydropterin [25-glutamine] (human clone lamdaHSY2 gene PCBD subunit), synthase, 6-pyruvoyltetrahydropterin [87-leucine] (human clone lamdaHSY2 gene PCBD subunit), synthase, 6-pyruvoyltetrahydropterin [de-57-valine] (human clone lambdaHSY2 gene PCBD subunit)

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.12 6-pyruvoyltetrahydropterin synthase

Engineering

Engineering on EC 4.2.3.12 - 6-pyruvoyltetrahydropterin synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C10A
-
50% decrease in activity
D96N
-
mutant enzyme that causes PTPS deficiency
Delta1-11
-
Flag-tag fused PTPS with N-terminal deletion, 97% decrease in activity
DELTA119-145
-
Flag-tag fused PTPS with C-terminal deletion, no detectable activity, no more nuclear staining
DELTA143-145
-
Flag-tag fused PTPS with C-terminal deletion, no detectable activity, no more nuclear staining
DELTAV57
-
mutant enzyme DELTAV57 is incorrectly folded and thus unstable
K143A
-
35% decrease in activity
P87L
-
phosphorylated, 30% of activity of wild type enzyme
R25G
-
mutant enzyme that causes PTPS deficiency
R87L
-
has substantial activity but enhanced sensitivity to local unfolding
T106M
-
mutant enzyme that causes PTPS deficiency
V56M
-
mutant enzyme that causes PTPS deficiency
V70D
-
mutant enzyme that causes PTPS deficiency
C42A
-
no catalytic activity, complete loss of metal binding site and activity, it is the only Cys in the active site
E133Q
-
1.3% of wild-type activity but similar affinity for the substrate
H23L
-
complete loss of metal binding site and activity
H48L
-
complete loss of metal binding site and activity
H50L
-
complete loss of metal binding site and activity
H89N
-
4.3% of wild-type activity but similar affinity for the substrate
additional information