4.2.3.119: (-)-alpha-pinene synthase

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For detailed information about (-)-alpha-pinene synthase, go to the full flat file.

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Synonyms

(-)-alpha-pinene cyclase, (-)-alpha-pinene/(-)-camphene synthase, (-)-limonene/(-)-alpha-pinene synthase, (-)-pinene cyclase, (-)-pinene synthase, ag6, ag9, alpha pinene sythase, alpha-pinene sythase, AvPS, AvTPS1, camphene synthase, cyclase II, EC 4.1.99.8, EC 4.2.3.14, LPS, mono-tps, More, pinene synthase, pinene-synthase, PlPIN, PT1, QH6, TPS-(-)apin1, TPS-(-)bpin2, TPS1, TPS2, TPS3

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.119 (-)-alpha-pinene synthase

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Results	in table
2	Activating Compound
35	AA Sequence
1	Application
1	CAS Registry Number
11	Cloned(Commentary)
1	Crystallization (Commentary)
5	Expression
10	General Information
4	IC50 Value
13	Inhibitors
6	Ki Value [mM]
14	KM Value [mM]
3	Localization
18	Metals/Ions
9	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
30	Organism
6	Pathway
5	pH Optimum
6	pH Range
2	pI Value
4	Posttranslational Modification
20	Protein Variants
3	Purification (Commentary)
1	Reaction
27	Reference
1	Renatured (Commentary)
16	Source Tissue
1	Storage Stability
50	Substrates and Products (Substrate)
6	Subunits
37	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Systematic Name

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Systematic Name on EC 4.2.3.119 - (-)-alpha-pinene synthase

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SYSTEMATIC NAME

IUBMB Comments

geranyl-diphosphate diphosphate-lyase [cyclizing, (-)-alpha-pinene-forming]

Cyclase II of Salvia officinalis (sage) gives about equal parts (-)-alpha-pinene, (-)-beta-pinene and (-)-camphene, plus traces of other monoterpenoids. (3S)-Linalyl diphosphate can also be used by the enzyme in preference to (3R)-linalyl diphosphate. The 4-pro-S-hydrogen of geranyl diphosphate is lost. Requires Mg2+ (preferred to Mn2+) [1-6]. The enzyme from Abies grandis (grand fir) gives roughly equal parts (-)-alpha-pinene and (-)-beta-pinene. However the clone ag11 gave 35% (-)-limonene, 24% (-)-alpha-pinene and 20% (-)-beta-phellandrene. It requires Mn2+ and K+ (Mg2+ is ineffective) [7-10]. Synthase I from Pinus taeda (loblolly pine) produces (-)-alpha-pinene with traces of (-)-beta-pinene and requires Mn2+ (preferred to Mg2+) [11,12]. The enzyme from Picea sitchensis (Sika spruce) forms 70% (-)-alpha-pinene and 30% (-)-beta-pinene [13]. The recombinant PmeTPS1 enzyme from Pseudotsuga menziesii (Douglas fir) gave roughly equal proportions of (-)-alpha-pinene and (-)-camphene plus traces of other monoterpenoids [14]. See also EC 4.2.3.120, (-)-beta-pinene synthase; EC 4.2.3.117, (-)-camphene synthase; EC 4.2.3.16, (-)-limonene synthase; and EC 4.2.3.52, (-)-beta-phellandrene synthase.