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4.2.3.117: (-)-camphene synthase

This is an abbreviated version!
For detailed information about (-)-camphene synthase, go to the full flat file.

Word Map on EC 4.2.3.117

Reaction

geranyl diphosphate
=
(-)-camphene
+
diphosphate

Synonyms

(-)-alpha-pinene/(-)-camphene synthase, (-)-pinene synthase, AaTPS5, ag6, camphene synthase, cl9841, cyclase II, More, TPS1

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.117 (-)-camphene synthase

Cloned

Cloned on EC 4.2.3.117 - (-)-camphene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AaTPS5, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme, lacking the N-terminal signal peptide, in Escherichia coli strain BL21(DE3)
expression in Escherichia coli
gene cl9841, the TPS gene identified, transcript cl9841, is a cluster of 9 sequences but only one is expressed in leaves and flowers. The protein shares 78% similarity with Solanum lycopersicum TPS3 protein, a monoterpene (-)-camphene/tricyclene synthase (UniProt ID G1JUH1) and a putative (-)-camphene/tricyclene synthase from Solanum tuberosum, DNA and amino acid sequence determination and analysis, recombinant coexpression with copalyl diphosphate synthase from Salvia pomifera and Saccharomyces cerevisiae ERG20 mutant F96C producing GGPP in Saccharomyces cerevisiae strain AM 238
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