4.2.3.114: gamma-terpinene synthase
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For detailed information about gamma-terpinene synthase, go to the full flat file.
Reaction
Synonyms
CitMTSL61, ClcTS, Os04g27790, OsTPS24, OvTPS2, RPS2, terpene synthase 24, TPS1, TPS2, TPS4, TvTPS, TvTPS1, TvTPS2
ECTree
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Crystallization
Crystallization on EC 4.2.3.114 - gamma-terpinene synthase
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homology modeling based on gamma-terpinene synthase from Origanum vulgare. Besides the aspartate-rich region DDxxD, the sites Asp452, Thr456 and Glu460 involved on stabilization of active site by Mg2+ cofactor are also conserved
R4JHV6
purified recombinant enzyme, sitting drop method, mixing of 400-600 nl of 10 mg/ml protein in 20 mM Tris-HCl, pH 7.8, 1 mM MgCl2, 4 mM DTT, with reservoir solution containing 0.2 M ammonium tartrate, pH 7.0, 20% w/v PEG 3350, in a 1:1 or 1:2 ratio, equilibration against 0.07 ml reservoir solution, 19°C, X-ray diffraction structure determination and analysis at 1.65-1.90 A resolution, two molecules (monomers A and B) in the asymmetric unit, molecular replacement using the crystal structure of (4S)-limonene synthase from Mentha spicata, PDB ID 2ong, model building