4.2.3.114: gamma-terpinene synthase
This is an abbreviated version!
For detailed information about gamma-terpinene synthase, go to the full flat file.
Word Map on EC 4.2.3.114
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Synonyms
CitMTSL61, ClcTS, Os04g27790, OsTPS24, OvTPS2, RPS2, terpene synthase 24, TPS1, TPS2, TPS4, TvTPS, TvTPS1, TvTPS2
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Cloned on EC 4.2.3.114 - gamma-terpinene synthase
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene CitMTSL61, HA-tag and a start codon are introduced to the synthetic gene before the arginine pair at position R56-R57 by using PCR to create gTerpS, recombinant overexpression of the codon-optimized gTerpS from plasmid pHHO5 in Aspergillus nidulans strain A772 resulting in production of gamma-terpinene, and expression of codon-optimized gTerpS in Saccharomyces cerevisiae strain H2802 from vector B1181. Real-time quantitative reverse transcription PCR expression analysis. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds
gene OsTPS24, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, functional recombinant expression of His-tagged recombinant TPS24 lacking the putative chloroplast transit sequence in Escherichia coli, expression of GFP-tagged enzyme in Oryza sativa protoplasts chloroplasts
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gene TvTPS, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 CodonPlus
real-time PCR enzyme expression analysis
