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4.2.2.7: heparin lyase

This is an abbreviated version!
For detailed information about heparin lyase, go to the full flat file.

Word Map on EC 4.2.2.7

Reaction

Eliminative cleavage of polysaccharides containing (1->4)-linked D-glucuronate or L-iduronate residues and (1->4)-alpha-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends =

Synonyms

Hep III, HepA, heparin degrading enzyme, Heparin eliminase, heparin lyase, heparin lyase 1, heparin lyase I, heparin lyase II, Heparinase, heparinase I, heparinase II, heparitinase II, heparitinase III, HepI, HepII, HepP, HMPREF1016_02668, Lyase, heparin, PA14_23430, Pedsa_1818, PL12a

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.7 heparin lyase

Reference

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Reference on EC 4.2.2.7 - heparin lyase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, V.C.; Linhard, R.J.; Bernstein, H.; Cooney, Ch.L.; Langer, R.
Purification and characterization of heparinase from Flavobacterium heparinum
J. Biol. Chem.
260
1849-1857
1985
Pedobacter heparinus
Manually annotated by BRENDA team
Nakamura, T.; Shibata, Y.; Fujimura, S.
Purification and properties of Bacteroides heparinolyticus heparinase (heparin lyase, EC 4.2.2.7)
J. Clin. Microbiol.
26
1070-1071
1988
Bacteroides heparinolyticus
Manually annotated by BRENDA team
Silverberg, I.; Havsmark, B.; Fransson, L.A.
The substrate specificity of heparan sulphate lyase and heparin lyase from Flavobacterium heparinum
Carbohydr. Res.
137
227-238
1985
Pedobacter heparinus
-
Manually annotated by BRENDA team
Arbogast, B.; Hopwood, J.J.; Dorfman, A.
Heparinase activity in rat liver
Biochem. Biophys. Res. Commun.
75
610-617
1977
Rattus norvegicus
Manually annotated by BRENDA team
Silva, M.E.; Dietrich, C.P.
Structure of heparin. Characterization of the products formed from heparin by the action of a heparinase and a heparitinase from Flavobacterium heparinum
J. Biol. Chem.
250
6841-6846
1975
Pedobacter heparinus
Manually annotated by BRENDA team
Dietrich, C.P.; Silva, M.E.; Michelacci, Y.M.
Sequential degradation of heparin in Flavobacterium heparinum. Purification and properties of five enzymes involved in heparin degradation
J. Biol. Chem.
248
6408-6415
1973
Pedobacter heparinus
Manually annotated by BRENDA team
Hovingh, P.; Linker, A.
The enzymatic degradation of heparin and heparitin sulfate. 3. Purification of a heparitinase and a heparinase from flavobacteria
J. Biol. Chem.
245
6170-6175
1970
Flavobacteriia
Manually annotated by BRENDA team
Yang, V.C.; Bernstein, H.; Langer, R.
Large scale production of heparinase
Biotechnol. Prog.
3
27-30
1987
Pedobacter heparinus
-
Manually annotated by BRENDA team
Linhardt, R.J.; Fitzgerald, G.L.; Cooney, Ch.L.
Mode of action of heparin lyase on heparin
Biochim. Biophys. Acta
702
197-203
1982
Pedobacter heparinus
Manually annotated by BRENDA team
Bernstein, H.; Yang, V.C.; Langer, R.
An investigation of heparinase immobilization
Appl. Biochem. Biotechnol.
16
129-143
1987
Pedobacter heparinus
Manually annotated by BRENDA team
Linhardt, R.J.; Cooney, C.L.; Tapper, D.; Zannetos, C.A.; Larsen, A.K.; Langer, R.
An immobilized microbial heparinase for blood deheparinization
Appl. Biochem. Biotechnol.
9
41-55
1984
Pedobacter heparinus
Manually annotated by BRENDA team
Joubert, J.J.; Pitout, M.J.
A constitutive heparinase in a Flavobacterium sp.
Experientia
41
1541
1985
Flavobacterium sp.
-
Manually annotated by BRENDA team
Cerbelaud, E.C.; Conway, L.J.; Galliher, P.M.; Langer, R.S.; Cooney, C.L.
Sulfur regulation of heparinase and sulfatases in Flavobacterium heparinum
Appl. Environ. Microbiol.
51
640-646
1986
Pedobacter heparinus
Manually annotated by BRENDA team
Bernstein, H.; Yang, V.C.; Langer, R.
Distribution of heparinase covalently immobilized to agarose: Experimental and theoretical studies
Biotechnol. Bioeng.
30
196-207
1987
Pedobacter heparinus
Manually annotated by BRENDA team
Yang, V.C.; Bernstein, H.; Cooney, Ch.L.; Langer, R.
Large scale preparation and characterization of mucopolysaccharase contamination free heparinase
Appl. Biochem. Biotechnol.
16
35-50
1987
Pedobacter heparinus
Manually annotated by BRENDA team
Yoshida, E.; Sakai, K.; Tokuyama, S.; Miyazono, H.; Maruyama, H.; Morikawa, K.; Yoshida, K.; Tahara, Y.
Purification and characterization of heparinase that degrades both heparin and heparan sulfate from Bacillus circulans
Biosci. Biotechnol. Biochem.
66
1181-1184
2002
Niallia circulans, Niallia circulans HpT298
Manually annotated by BRENDA team
Yoshida, E.; Arakawa, S.; Matsunaga, T.; Toriumi, S.; Tokuyama, S.; Morikawa, K.; Tahara, Y.
Cloning, sequencing, and expression of the gene from Bacillus circulans that codes for a heparinase that degrades both heparin and heparan sulfate
Biosci. Biotechnol. Biochem.
66
1873-1879
2002
Niallia circulans (Q8KKH9), Niallia circulans, Niallia circulans HpT298 (Q8KKH9)
Manually annotated by BRENDA team
Kim, B.T.; Kim, W.S.; Kim, Y.S.; Linhardt, R.J.; Kim, D.H.
Purification and characterization of a novel heparinase from Bacteroides stercoris HJ-15
J. Biochem.
128
323-328
2000
Bacteroides stercoris, Bacteroides stercoris HJ-15
Manually annotated by BRENDA team
Yapeng, C.; Ningguo, G.; Xiulan, C.; Jing, Y.; Shijun, Q.; Shuzheng, Z.
Rapid purification, characterization and substrate specificity of heparinase from a novel species of Sphingobacterium
J. Biochem.
134
365-371
2003
Sphingobacterium sp.
Manually annotated by BRENDA team
Liu, D.; Shriver, Z.; Godavarti, R.; Venkataraman, G.; Sasisekharan, R.
The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymic activity
J. Biol. Chem.
274
4089-4095
1999
Pedobacter heparinus
Manually annotated by BRENDA team
Yu, G.; LeBrun, L.; Gunay, N.S.; Hoppensteadt, D.; Walenga, J.M.; Fareed, J.; Linhardt, R.J.
Heparinase I acts on a synthetic heparin pentasaccharide corresponding to the antithrombin III binding site
Thromb. Res.
100
549-556
2000
Pedobacter heparinus
Manually annotated by BRENDA team
Shaya, D.; Li, Y.; Cygler, M.
Crystallization and preliminary X-ray analysis of heparinase II from Pedobacter heparinus
Acta Crystallogr. Sect. D
60
1644-1646
2004
Pedobacter heparinus
Manually annotated by BRENDA team
Chem, Y.; Xing, X.H.; Lou, K.
Construction of recombinant Escherichia coli for over-production of soluble heparinase I by fusion to maltose-binding protein
Biochem. Eng. J.
23
155-159
2005
Pedobacter heparinus
-
Manually annotated by BRENDA team
Kim, W.S.; Kim, B.T.; et.al.
Purification of heparin lyase I from Bacteroides stercoris HJ-15
J. Biochem. Mol. Biol.
37
684-690
2004
Bacteroides stercoris, Bacteroides stercoris HJ-15
Manually annotated by BRENDA team
Wei, Z.; Lyon, M.; Gallagher, J.T.
Distinct substrate specificities of bacterial heparinases against N-unsubstituted glucosamine residues in heparan sulfate
J. Biol. Chem.
280
15742-15748
2005
Pedobacter heparinus
Manually annotated by BRENDA team
Shaya, D.; Tocilj, A.; et.al.
Crystal structure of heparinase II from Pedobacter heparinus and its complex with a disaccharide product
J. Biol. Chem.
281
15525-15535
2006
Pedobacter heparinus
Manually annotated by BRENDA team
Luo, Y.; Huang, X.; McKeehan, W.L.
High yield, purity and activity of soluble recombinant Bacteroides thetaiotaomicron GST-heparinase I from Escherichia coli
Arch. Biochem. Biophys.
460
17-24
2007
Bacteroides thetaiotaomicron
Manually annotated by BRENDA team
Ma, X.; Wang, Z.; Li, S.; Shen, Q.; Yuan, Q.
Effect of CaCl2 as activity stabilizer on purification of heparinase I from Flavobacterium heparinum
J. Chromatogr. B
843
209-215
2006
Pedobacter heparinus
Manually annotated by BRENDA team
Ahn, S.C.; Kim, B.Y.; Oh, W.K.; Park, Y.M.; Kim, H.M.; Ahn, J.S.
Colorimetric heparinase assay for alternative anti-metastatic activity
Life Sci.
79
1661-1665
2006
Pedobacter heparinus
Manually annotated by BRENDA team
Manton, K.J.; Leong, D.F.; Cool, S.M.; Nurcombe, V.
Disruption of heparan and chondroitin sulfate signaling enhances mesenchymal stem cell-derived osteogenic differentiation via bone morphogenetic protein signaling pathways
Stem Cells
25
2845-2854
2007
Homo sapiens
Manually annotated by BRENDA team
Banga, J.; Tripathi, C.K.; Bihari, V.
Growth and enzyme production kinetics of a heparinase-producing fungal isolate
Med. Chem. Res.
17
85-93
2008
Aspergillus oryzae, Pedobacter heparinus
-
Manually annotated by BRENDA team
Hyun, Y.J.; Lee, K.S.; Kim, D.H.
Cloning, expression and characterization of acharan sulfate-degrading heparin lyase II from Bacteroides stercoris HJ-15
J. Appl. Microbiol.
108
226-235
2010
Bacteroides stercoris (C0JBW5), Bacteroides stercoris HJ-15 (C0JBW5), Bacteroides stercoris HJ-15
Manually annotated by BRENDA team
Han, Y.H.; Garron, M.L.; Kim, H.Y.; Kim, W.S.; Zhang, Z.; Ryu, K.S.; Shaya, D.; Xiao, Z.; Cheong, C.; Kim, Y.S.; Linhardt, R.J.; Jeon, Y.H.; Cygler, M.
Structural snapshots of heparin depolymerization by heparin lyase I
J. Biol. Chem.
284
34019-34027
2009
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron WAL2926
Manually annotated by BRENDA team
Shaya, D.; Zhao, W.; Garron, M.L.; Xiao, Z.; Cui, Q.; Zhang, Z.; Sulea, T.; Linhardt, R.J.; Cygler, M.
Catalytic mechanism of heparinase II investigated by site-directed mutagenesis and the crystal structure with its substrate
J. Biol. Chem.
285
20051-20061
2010
Pedobacter heparinus
Manually annotated by BRENDA team
Banga, J.; Tripathi, C.K.M.
Purification and characterization of a novel heparin degrading enzyme from Aspergillus flavus (MTCC-8654)
Appl. Biochem. Biotechnol.
160
1004-1016
2010
Aspergillus flavus, Aspergillus flavus MTCC-8654
Manually annotated by BRENDA team
Raman, K.; Kuberan, B.
Differential effects of heparitinase I and heparitinase III on endothelial tube formation in vitro
Biochem. Biophys. Res. Commun.
398
191-193
2010
Pedobacter heparinus
Manually annotated by BRENDA team
Zhao, W.; Garron, M.L.; Yang, B.; Xiao, Z.; Esko, J.D.; Cygler, M.; Linhardt, R.J.
Asparagine 405 of heparin lyase II prevents the cleavage of glycosidic linkages proximate to a 3-O-sulfoglucosamine residue
FEBS Lett.
585
2461-2466
2011
Pedobacter heparinus
Manually annotated by BRENDA team
Xiao, Z.; Zhao, W.; Yang, B.; Zhang, Z.; Guan, H.; Linhardt, R.J.
Heparinase 1 selectivity for the 3,6-di-O-sulfo-2-deoxy-2-sulfamido-alpha-D-glucopyranose (1,4) 2-O-sulfo-alpha-L-idopyranosyluronic acid (GlcNS3S6S-IdoA2S) linkages
Glycobiology
21
13-22
2011
Pedobacter heparinus
Manually annotated by BRENDA team
Wu, J.; Zhang, C.; Mei, X.; Li, Y.; Xing, X.H.
Controllable production of low molecular weight heparins by combinations of heparinase I/II/III
Carbohydr. Polym.
101
484-492
2014
Pedobacter heparinus
Manually annotated by BRENDA team
Cordula, C.R.; Lima, M.A.; Shinjo, S.K.; Gesteira, T.F.; Pol-Fachin, L.; Coulson-Thomas, V.J.; Verli, H.; Yates, E.A.; Rudd, T.R.; Pinhal, M.A.; Toma, L.; Dietrich, C.P.; Nader, H.B.; Tersariol, I.L.
On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+
Mol. Biosyst.
10
54-64
2014
Pedobacter heparinus, Pedobacter heparinus ATCC 13125
Manually annotated by BRENDA team
Huang, J.; Cao, L.; Guo, W.; Yuan, R.; Jia, Z.; Huang, K.
Enhanced soluble expression of recombinant Flavobacterium heparinum heparinase I in Escherichia coli by fusing it with various soluble partners
Protein Expr. Purif.
83
169-176
2012
Pedobacter heparinus
Manually annotated by BRENDA team
Balasubramaniam, K.; Sharma, K.; Rani, A.; Rajulapati, V.; Goyal, A.
Deciphering the mode of action, structural and biochemical analysis of heparinase II/III (PsPL12a) a new member of family 12 polysaccharide lyase from Pseudopedobacter saltans
Ann. Microbiol.
68
409-418
2018
Pseudopedobacter saltans (F0S8P2), Pseudopedobacter saltans DSM 12145 (F0S8P2)
-
Manually annotated by BRENDA team
Yu, P.; Jia, T.; Chen, Y.; Wu, Y.; Zhang, Y.
Improving the activity of heparinase I by the directed evolution, its enzymatic properties and optimal conditions for heparin degrading by recombinant cells
Biochem. Eng. J.
114
237-243
2016
Pedobacter heparinus (B3U3D9)
-
Manually annotated by BRENDA team
Yang, M.; Chen, J.; Zhou, H.; Li, W.; Wang, Y.; Li, J.; Zhang, C.; Zhou, C.; Yu, C.
Polycation-induced benzoperylene probe excimer formation and the ratiometric detection of heparin and heparinase
Biosens. Bioelectron.
75
404-410
2016
Pedobacter heparinus
Manually annotated by BRENDA team
Yang, B.; Zhang, C.; Wang, C.; Zhou, H.; Li, Z.; Song, Y.; Zhang, T.; Luo, X.
Soluble expression and purification of heparinase I in Escherichia coli using a hexahistidine-tagged small ubiquitin-like modifier as a fusion partner
Biotechnol. Biotechnol. Equip.
31
1040-1045
2017
Pedobacter heparinus
-
Manually annotated by BRENDA team
Dzvova, N.; Colmer-Hamood, J.A.; Griswold, J.A.; Hamood, A.N.
Isolation and characterization of HepP a virulence-related Pseudomonas aeruginosa heparinase
BMC Microbiol.
17
233
2017
Pseudomonas aeruginosa (A0A0H2ZDM9), Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14 (A0A0H2ZDM9)
Manually annotated by BRENDA team
Bohlmann, L.; Chang, C.W.; Beacham, I.; von Itzstein, M.
Exploring bacterial heparinase II activities with defined substrates
ChemBioChem
16
1205-1211
2015
Pedobacter heparinus, Bacteroides eggerthii (E5X103), Bacteroides eggerthii, Bacteroides eggerthii 1_2_48FAA (E5X103)
Manually annotated by BRENDA team
Sefrioui, D.; Beaussire, L.; Clatot, F.; Delacour, J.; Perdrix, A.; Frebourg, T.; Michel, P.; Di Fiore, F.; Sarafan-Vasseur, N.
Heparinase enables reliable quantification of circulating tumor DNA from heparinized plasma samples by droplet digital PCR
Clin. Chim. Acta
472
75-79
2017
Bacteroides eggerthii
Manually annotated by BRENDA team
Dzvova, N.; Colmer-Hamood, J.A.; Griswold, J.A.; Hamood, A.N.
Heparinase is essential for Pseudomonas aeruginosa virulence during thermal injury and infection
Infect. Immun.
86
e00755
2018
Pseudomonas aeruginosa (A0A0H2ZDM9), Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14 (A0A0H2ZDM9)
Manually annotated by BRENDA team
Xu, S.; Qiu, M.; Zhang, X.; Chen, J.
Expression and characterization of an enhanced recombinant heparinase I with chitin binding domain
Int. J. Biol. Macromol.
105
1250-1258
2017
Pedobacter heparinus (B3U3D9)
Manually annotated by BRENDA team