4.2.2.23: rhamnogalacturonan endolyase
This is an abbreviated version!
For detailed information about rhamnogalacturonan endolyase, go to the full flat file.
Word Map on EC 4.2.2.23
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4.2.2.23
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polysaccharide
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aculeatus
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lyases
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homogalacturonans
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beta-elimination
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pectic
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hairy
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nonreducing
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rhamnogalacturonan-i
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mucilage
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d-galacturonic
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galactan
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arabinofuranosidase
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chrysogenum
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pectate
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l-rhamnose
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acetylesterase
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endo-acting
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reaction-generated
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camv35s
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ramified
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pectinases
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food industry
- 4.2.2.23
- polysaccharide
- aculeatus
- lyases
- homogalacturonans
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beta-elimination
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pectic
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hairy
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nonreducing
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rhamnogalacturonan-i
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mucilage
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d-galacturonic
- galactan
- arabinofuranosidase
- chrysogenum
- pectate
- l-rhamnose
- acetylesterase
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endo-acting
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reaction-generated
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camv35s
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ramified
- pectinases
- food industry
Reaction
Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. =
Synonyms
101259106, alpha-L-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronide lyase, RG lyase, RG-lyase, RGase B, Rgl11A, Rgl11Y, RGL4, Rgl4A, RglA, RglF, rhamnogalacturonan alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galactopyranosyluronide lyase, rhamnogalacturonan lyase A, rhamnogalacturonase B, RhiE, Solyc11g011300, YesW
ECTree
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KM Value
KM Value on EC 4.2.2.23 - rhamnogalacturonan endolyase
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additional information
Km value 0.74 mg/ml, pH 7.0, 37°C
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additional information
degalactosylated rhamnogalacturonan
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Km value 0.74 mg/ml, pH 7.0, 37°C
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additional information
pectic galactan
Km value of wild-type 10 mg/ml, of truncated mutant 11.2 mg/ml, respectively, pH 8.5, 70°C
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additional information
Pectin
Km value of wild-type 8.2 mg/ml, of truncated mutant 7.6 mg/ml, respectively, pH 8.5, 70°C
additional information
potato galactan
Km value of wild-type 4.7 mg/ml, of truncated mutant 6.4 mg/ml, respectively, pH 8.5, 70°C
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additional information
potato pectic galactan
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Km-value: 1 g/l, pH 8.5, 37°C
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additional information
potato pectic galactan
KM-value: 8.5 mg/ml at pH 9.5
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additional information
potato pectic galactan
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KM-value: 8.5 mg/ml at pH 9.5
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additional information
potato rhamnogalacturonan I
Km value of wild-type 4.9 mg/ml, of truncated mutant 6.0 mg/ml, respectively, pH 8.5, 70°C
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additional information
rhamnogalacturonan
Km value 1.67 mg/ml, pH 7.0, 37°C
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additional information
rhamnogalacturonan
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Km value 1.67 mg/ml, pH 7.0, 37°C
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additional information
rhamnogalacturonan I
KM-value is 0.181 mg/ml, pH 7.5, 30°C
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additional information
rhamnogalacturonan I
Km-value is 0.35 mg/ml, pH 7.5, 30°C
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additional information
rhamnogalacturonan I
Km-value is 1.3 mg/ml or 0.27 mM cleavable bonds, pH 6.0, 30°C
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additional information
rhamnogalacturonan I
KM-value: 0.13 mg/ml for wild-type enzyme, 1.9 mg/ml for mutant enzyme K535A, 2.5 mg/ml for mutant enzyme R452A, 0.033 mg/ml for mutant enzyme Y595F, 0.1 mg/ml for mutant enzymes D401N and H363A, 0.12 mg/mL for mutant enzyme H399A, pH 7.5, 30°C
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additional information
rhamnogalacturonan I
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KM-value: 0.13 mg/ml for wild-type enzyme, 1.9 mg/ml for mutant enzyme K535A, 2.5 mg/ml for mutant enzyme R452A, 0.033 mg/ml for mutant enzyme Y595F, 0.1 mg/ml for mutant enzymes D401N and H363A, 0.12 mg/mL for mutant enzyme H399A, pH 7.5, 30°C
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additional information
rhamnogalacturonan I
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KM-values of recombinant rhamnogalacturonan lyase towards enzyme-treated saponified modified hairy regions of pectin
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additional information
soybean rhamnogalacturonan I
Km value of wild-type 4.8 mg/ml, of truncated mutant 5.1 mg/ml, respectively, pH 8.5, 70°C
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