4.2.2.19: chondroitin B lyase

This is an abbreviated version!
For detailed information about chondroitin B lyase, go to the full flat file.

+ 4-deoxy-beta-D-gluc-4-enuronosyl-1,3-GalNAc

ChnB, ChonB, chondroitin B lyase, chondroitinase B, CslB, lyase, chondroitin B

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Results	in table
1	Activating Compound
71	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
3	Crystallization (Commentary)
14	Disease/ Diagnostics
1	General Stability
27	Inhibitors
2	Ki Value [mM]
10	KM Value [mM]
1	Localization
2	Metals/Ions
3	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
16	Organism
3	Pathway
5	pH Optimum
1	pI Value
1	Posttranslational Modification
12	Protein Variants
4	Purification (Commentary)
4	Reaction
1	Reaction Type
16	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
19	Substrates and Products (Substrate)
3	Subunits
18	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
7	Turnover Number [1/s]

Purification on EC 4.2.2.19 - chondroitin B lyase

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purification based on affinity chromatography

recombinant enzyme comprising residues 25-506 overexpressed in Flavobacterium heparinum cells, to homogeneity

recombinant His-tagged enzyme from Escherichia coli, His-tag is removed, purified to homogeneity, 69fold

to homogeneity