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4.2.2.19: chondroitin B lyase

This is an abbreviated version!
For detailed information about chondroitin B lyase, go to the full flat file.

Word Map on EC 4.2.2.19

Reaction

[dermatan sulfate]n
=
[dermatan sulfate]n-1
 + 4-deoxy-beta-D-gluc-4-enuronosyl-1,3-GalNAc

Synonyms

ChnB, ChonB, chondroitin B lyase, chondroitinase B, CslB, lyase, chondroitin B

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.19 chondroitin B lyase

Engineering

Engineering on EC 4.2.2.19 - chondroitin B lyase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E243A
site-directed mutagenesis, Ca2+-binding residue, about 3.6fold reduced activity compared to the wild-type enzyme
E243A/E245A
site-directed mutagenesis, Ca2+-binding residue, about 4fold reduced activity compared to the wild-type enzyme
E245A
site-directed mutagenesis, Ca2+-binding residue, about 6fold reduced activity compared to the wild-type enzyme
E333A
PCR site-directed mutagenesis, E333 is a key residue in catalysis, reduced activity
H272A
PCR site-directed mutagenesis, H272 is a key residue in catalysis, reduced activity
K250A
PCR site-directed mutagenesis, inactive mutant
N213Q
site-directed mutagenesis, Ca2+-binding residue, about 6fold reduced activity compared to the wild-type enzyme
R271A
PCR site-directed mutagenesis, R271 is a key residue in catalysis
R271E
site-directed mutagenesis, active site mutant, catalytically inactive
R271K
site-directed mutagenesis, active site mutant, about 10fold reduced activity compared to the wild-type enzyme
R363A
PCR site-directed mutagenesis, R363 is a key residue in catalysis, 2fold increased activity
R364A
PCR site-directed mutagenesis, highly reduced activity, altered product profil, residue is involved in determination of substrate specificity