4.2.2.14: glucuronan lyase

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For detailed information about glucuronan lyase, go to the full flat file.

Word Map on EC 4.2.2.14

4.2.2.14

glucuronans

trichoderma

polysaccharide

meliloti

depolymerization

synthesis

unsaturated

reesei

oligosaccharides

sinorhizobium

oligomers

ncimb

chitinase

4,5-unsaturated

glucuronic

alginate

deacetylated

glycoside

biotechnology

Reaction

= n-1 4-deoxy-beta-D-gluc-4-enuronosyl-[1,4-beta-D-GlcA]3 +

Synonyms

(1,4)-beta-D-glucuronan lyase, (1->4)-beta-D-glucuronan lyase, endo-beta-(1->4)-glucuronan lyase, endo-beta-1,4-glucuronan lyase, endopolyglucuronate lyase, GL2 glucuronan lyase, glucuronan lyase, TrGL

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.2 Acting on polysaccharides

4.2.2.14 glucuronan lyase

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Results	in table
2	AA Sequence
8	Application
1	CAS Registry Number
2	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Stability
16	Inhibitors
2	Localization
8	Metals/Ions
3	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
41	Organism
1	PDB ID
4	pH Optimum
3	pH Stability
2	pI Value
4	Purification (Commentary)
3	Reaction
1	Reaction Type
19	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
21	Substrates and Products (Substrate)
4	Subunits
14	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
6	Temperature Stability [°C]

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biotechnology

Trichoderma sp.

a glucuronan lyase is immobilized on a monolithic Convective Interaction Media disk. Degradations of three glucuronans with various O-acetylation degrees is investigated and compared with degradations using free enzyme. The immobilized glucuronan lyase is inhibited by the O-acetylation degree like the free enzyme. 1H NMR analyses are used to study the O-acetylation degree of oligoglucuronans and demonstrate that the average degrees of polymerization are inclusive between 4 and 13 after 24 h of degradation. This first immobilization of a glucuronan lyase constitutes a tool to produce oligoglucuronans

691646

synthesis

7 entries