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4.2.2.11: guluronate-specific alginate lyase

This is an abbreviated version!
For detailed information about guluronate-specific alginate lyase, go to the full flat file.

Word Map on EC 4.2.2.11

Reaction

[alpha-1,4-L-guluronate]n
=
alpha-L-guluronate
+
4-deoxy-alpha-L-erythro-hex-4-enuronosyl-[alpha-1,4-L-guluronate]n-2

Synonyms

A1m, A9mC, A9mL, A9mT, Alg17C, Alg2A, Alg7D, AlgB, alginase II, alginate (poly-alpha-L-guluronate) lyase, alginate lyase, AlgL, AlgL5, AlgMytC, alkaliphilic alginate lyase, ALY, Aly-SJ02, Aly1, Aly5, AlyA, AlyA1PL7, AlyA5, AlyD, AlyDW11, AlyE, AlyQ, AlyV5, AXE80_11190, endo-type alginate lyase, guluronate lyase, KJ-2 alginate lyase, L-guluronan lyase, L-guluronate lyase, lyase, polyguluronate, Oal17A, oligoalginate lyase, poly(1,4-alpha-L-guluronide)lyase, poly(alpha-L-guluronate) lyase, poly-alpha-L-guluronate lyase, polyguluronate-specific alginate lyase, polyMG-specific alginate lyase, V12B01_09446, V12B01_24274, WP_053404615, xadAly7B

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.11 guluronate-specific alginate lyase

Molecular Weight

Molecular Weight on EC 4.2.2.11 - guluronate-specific alginate lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23000
25000
25009
x * 25009, calculated
25270
-
MALDI-TOF mass spectrometry
26000
-
gel filtration
27000
-
gel filtration
28000
28400
-
amino acid analysis
29643
1 * 29643, calculated, 1 * 28000, SDS-PAGE
31000
31600
-
1 * 31600, intracellular, SDS-PAGE
32000
32700
-
x * 32700, recombinant His-tagged enzyme without, SDS-PAGE
33900
-
x * 33900, SDS-PAGE
35200
gel filtration
36000
36500
gel filtration
37000
-
x * 37000, SDS-PAGE
37573
x * 37573, sequence calculation, x * 43000, recombinant His-tagged enzyme, SDS-PAGE
38000
38300
monomeric recombinant His-tagged enzyme, gel filtration
39000
-
Sephacryl S-200 HR column chromatography
40000 - 42000
-
-
41000
42000
x * 42000, recombinant His-tagged enzyme, SDS-PAGE
42400
x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated
43000
45000
-
x * 45000 plus x * 50000, SDS-PAGE
45500
x * 45500, recombinant enzyme with signal peptide, x * 42400, recombinant enzyme without signal peptide, calulated
47800
x * 47800, recombinant His-tagged enzyme, SDS-PAGE
50000
-
x * 45000 plus x * 50000, SDS-PAGE
54426
x * 54426, calculated
60250
-
x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE
69500
dimeric recombinant His-tagged enzyme, gel filtration
77500
x * 77500, SDS-PAGE of recombinant protein used to maltose binding protein
78000
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE
79100
x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE
79900
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE
80000
x * 79900, including signal peptide, x * 78000, without signal peptide, calculated. x * 80000, SDS-PAGE
81600
x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE