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(NH4)2SO4
-
relative activity: 409%
Ag+
1 mM, 131% of initial activity
CH3COO- NH4+
-
relative activity: 298%
CH3COONa
-
relative activity: 233%
CoCl2
-
relative activity: 102%
Cs+
0.2 M, 2500% of initial activity
CuSO4
-
relative activity: 93%
EDTA
-
relative activity: 14%
EGTA
-
relative activity: 14%
Fe2+
1 mM, 148% of initial activity
HgCl2
-
relative activity: 29%
K2HPO4
-
relative activity: 439%
KCl
-
relative activity: 521%
Li+
0.2 M, 1880% of initial activity
NaBr
-
relative activity: 712%
NaF
-
relative activity: 419%
NH4+
0.2 M, 2270% of initial activity
NH4Cl
-
relative activity: 549%
NiCl2
-
relative activity: 106%
PdCl2
-
relative activity: 5%
Rb+
0.2 M, 2500% of initial activity
Sr2+
-
1 mM, 124% of initial activity
SrCl2
-
relative activity: 54%
ZnCl2
-
relative activity: 111%
Ba2+
-
1 mM, 143% of initial activity
Ba2+
1 mM, 113% of initial activity
Ca2+
-
Ca2+
-
slight activation at 1 mM or 5 mM concentrations
Ca2+
AB489222
158% of initial activity
Ca2+
1 mM, 174% of initial activity, 10 mM, 135% of initial activity
Ca2+
-
essential cofactor, not only affects enzyme kinetics but also the final degree of conversion
Ca2+
-
10 mM, 111% of initial activity
Ca2+
-
1 mM, 136% of initial activity
Ca2+
-
50 mM, isoform A 117%, isoform B 131% of initial activity
Ca2+
-
involved in the formation of the active intermediate between the acidic uronates and amino acid side chains of the enzyme
Ca2+
required for activity, activates at 1 mM
Ca2+
-
1 mM, 120% of initial activity
Ca2+
1 mM, 125% of initial activity
Ca2+
1 mM, 135% of initial activity
Ca2+
1 mM, 1.15fold increase in enzymatic activity
Ca2+
1 mM, 1.3fold increase in enzymatic activity
Ca2+
at least 2 mM Ca2+ in the reaction mixture is essential for the activity
Ca2+
requires a divalent cation for activity, interaction between the enzyme and divalent cations takes place during substrate fixation and not before
CaCl2
-
relative activity: 76%
CaCl2
-
20% activation at 1 mM
Co2+
1 mM, 116% of initial activity
Co2+
-
1 mM, 123% of initial activity
Co2+
1 mM, 156% of initial activity
Cu2+
-
2 mM, 66% inhibition
Cu2+
1 mM, 18% of initial activity
K+
-
optimum activity at 0.25 M. At 2 M, about 70% residual activity
K+
5 mM, 148% of initial activity
K+
-
0.1-0.3 M, activation
K+
50 mM, 10% increase in activity
K+
-
1 mM, 117% of initial activity
K+
-
50 mM, isoform A 132%, isoform B 110% of initial activity
K+
activates at over 50 mM
K+
0.2 M, 2500% of initial activity
K+
1 mM, 136% of initial activity
Mg2+
-
Mg2+
-
1 mM, 11% activation
Mg2+
AB489222
173% of initial activity
Mg2+
1 mM, 168% of initial activity, 10 mM, 119% of initial activity
Mg2+
-
0.05-0.1 M: activation, higher concentrations cause lower activity or precipitation of alginate
Mg2+
50 mM, 2fold increase in activity
Mg2+
-
1 mM, 126% of initial activity
Mg2+
-
50 mM, isoform A 116%, isoform B 121%, isoform C 114% of initial activity
Mg2+
-
1 mM, 115% of initial activity
MgCl2
-
relative activity: 101%
MgCl2
-
15% activation at 1 mM
Mn2+
-
Mn2+
-
5 mM, 255% activity
Mn2+
-
1 mM, 116% of initial activity
Mn2+
-
1 mM, 112% of initial activity
MnCl2
-
relative activity: 72%
MnCl2
-
12% activation at 1 mM
Na+
-
activity is increased to 1.8fold by adding 0.2 M NaCl
Na+
-
optimum activity at 0.25 M. At 2 M, about 70% residual activity
Na+
5 mM, 119% of initial activity
Na+
AB489222
optimum concentration 4 M, about 25% activation
Na+
100 mM, 126% of initial activity, 500 mM, 203% of initial activity
Na+
-
0.1-0.3 M, activation
Na+
-
concentrations higher than 3% w/w produce complete loss of activity, the activity is partially restored with 1 mM calcium
Na+
-
1 mM, 115% of initial activity
Na+
50 mM, 10% increase in activity
Na+
-
1 mM, 143% of initial activity
Na+
activates at over 50 mM
Na+
-
0,3-1 M optimum concentration
Na+
0.2 M, 2200% of initial activity
Na+
50 mM, 124% of initial activity
Na+
optimum concentration 0.3 M, about 2fold increase in activity compared to absence of NaCl
Na+
up to 10fold increase in activity, optimum concentration 400 mM
Na+
up to 3fold increase in activity, optimum concentration 400 mM
NaCl
-
relative activity: 823%
NaCl
-
2 mM, 110% of initial activity, 100 mM, 193% of initial activity
NaCl
activates, best at 150-200 mM
Ni2+
-
Ni2+
-
1 mM, 13% activation
Ni2+
-
1 mM, 110% of initial activity
Zn2+
-
Zn2+
-
1 mM, 175% activity
Zn2+
relevance of this zinc ion in catalytic activity, metal-binding protein ligands are His533, Gln551, and Lys573. The metal ion establishes the orientation of His415 and Arg438, which would otherwise be mobile, to set these residues for interactions with substrate
additional information
-
BaCl2 does not affect enzymatic activity
additional information
-
no effect on activity by K+
additional information
a metal ion is required. No or poor effects by SDS at 0.001-0.01%, and DTT, Na+ and K+ at 1-5 mM
additional information
-
no effect on enzyme activity by SDS, K+, Li+, and NH4+