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4.2.2.11: guluronate-specific alginate lyase

This is an abbreviated version!
For detailed information about guluronate-specific alginate lyase, go to the full flat file.

Word Map on EC 4.2.2.11

Reaction

[alpha-1,4-L-guluronate]n
=
alpha-L-guluronate
 +
4-deoxy-alpha-L-erythro-hex-4-enuronosyl-[alpha-1,4-L-guluronate]n-2

Synonyms

A1m, A9mC, A9mL, A9mT, Alg17C, Alg2A, Alg7D, AlgB, alginase II, alginate (poly-alpha-L-guluronate) lyase, alginate lyase, AlgL, AlgL5, AlgMytC, alkaliphilic alginate lyase, ALY, Aly-SJ02, Aly1, Aly5, AlyA, AlyA1PL7, AlyA5, AlyD, AlyDW11, AlyE, AlyQ, AlyV5, AXE80_11190, endo-type alginate lyase, guluronate lyase, KJ-2 alginate lyase, L-guluronan lyase, L-guluronate lyase, lyase, polyguluronate, Oal17A, oligoalginate lyase, poly(1,4-alpha-L-guluronide)lyase, poly(alpha-L-guluronate) lyase, poly-alpha-L-guluronate lyase, polyguluronate-specific alginate lyase, polyMG-specific alginate lyase, V12B01_09446, V12B01_24274, WP_053404615, xadAly7B

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.11 guluronate-specific alginate lyase

Cloned

Cloned on EC 4.2.2.11 - guluronate-specific alginate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
baculoviral expression system
-
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, phylogenetic analysis
AB489222
expressed in Escherichia coli
-
expression in Escherichia coli
expression in Escheriochia coli
gene alg17C, expression in Escherichia coli strain BL21(DE3)
gene alg2A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene alg7D, overexpression of soluble His-tagged enzyme lacking the signal peptide in Escherichia coli strain BL21(DE3)
-
gene algMytC, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) partly in inclusion bodies
phylogenetic analysis, recombinant expression of His-tagged AlyA1PL7 in Escherichia coli strain BL21(DE3)
phylogenetic analysis, recombinant expression of His-tagged AlyA5 in Escherichia coli strain BL21(DE3)
subcloning of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5-alpha, expression in Escherichia coli strain BL21(DE3) Rosetta, the enzymes are secreted from periplasmic space