4.2.1.95: kievitone hydratase
This is an abbreviated version!
For detailed information about kievitone hydratase, go to the full flat file.
Reaction
Synonyms
FsKHS, hydratase, kievitone, KHase, NhKHS
ECTree
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Cloned
Cloned on EC 4.2.1.95 - kievitone hydratase
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DNA and amino acid sequence determination and analysis, sequence comparisons, higher functional recombinant expression of codon-harmonized gene encoding the enzyme in Pichia pastoris (i.e. Komagataella phaffii) strain CBS7435 as C-terminally His10-tagged protein, the mature enzyme is secreted. Upscaling tests show that Pichia pastoris is a most appropriate host for producing high amounts of recombinant NhKHS
DNA and amino acid sequence determination and analysis, sequence comparisons, lower functional recombinant expression of codon-harmonized gene encoding the enzyme in Pichia pastoris (i.e. Komagataella phaffii) strain CBS7435 as C-terminally His10-tagged protein