4.2.1.94: scytalone dehydratase
This is an abbreviated version!
For detailed information about scytalone dehydratase, go to the full flat file.
Word Map on EC 4.2.1.94
-
4.2.1.94
-
melanin
-
fungicide
-
grisea
-
magnaporthe
-
dihydroxynaphthalene
-
pyricularia
-
tricyclazole
-
saga
-
lagenarium
-
cochliobolus
-
dhn-melanin
-
1,8-dihydroxynaphthalene
- 4.2.1.94
- melanin
-
fungicide
- grisea
-
magnaporthe
-
dihydroxynaphthalene
-
pyricularia
- tricyclazole
- saga
- lagenarium
- cochliobolus
-
dhn-melanin
- 1,8-dihydroxynaphthalene
Reaction
Synonyms
dehydratase, scytalone, Sal1, SCD, SCD1, scytalone dehydratase1, SS1G_13314
ECTree
Advanced search results
Engineering
Engineering on EC 4.2.1.94 - scytalone dehydratase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
V75M
-
inhibition of the mutant enzyme by (1RS,3RS)-2,2-dichloro-N-[(R)-1-(4-chlorophenyl)ethyl]-1-ethyl-3-methylcyclopropanecarboxamide is more than 200fold reduced in comparison with that of the wild-type, about 2fold reduced turnover number for scytalone as compared to wild-type enzyme
additional information
C-terminal deletion mutants: deletion of 6 amino acids, 95% loss of activity
additional information
-
inhibitors that display variations in the hydrophilic region of the SD binding site, kinetic data for wild type and mutant enzymes
additional information
-
fungal isolates resistant to the melanin biosynthesis inhibitors-dehydratase (MBI-D), which inhibits scytalone dehydratase, are detected in Japan. The isolates have a single point mutation in the gene encoding scytalone dehydratase. In fitness tests, resistant isolates show reduced fitness, compared with that of sensitive isolates. Enzyme kinetics analyses of the scytalone dehydratases from MBI-D-sensitive and MBI-D-resistant isolates reveal that the activity of MBI-D-sensitive-type enzymes is approximately double that of MBI-D-resistant-type enzymes. The sensitive-type enzyme has higher scytalone dehydration activity than that of resistant-type enzyme at temperatures ranging from 15-35°C and at pHs ranging from pH 6.0-9.0. Acquisition of resistance to MBI-D negatively affects the pathogenicity of Magnaporthe oryzae due to decreased scytalone dehydratase activity
additional information
gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods
additional information
-
gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods
additional information
Sclerotinia sclerotiorum ATCC 18683 / 1980 / Ss-1
-
gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods
-