4.2.1.93: ATP-dependent NAD(P)H-hydrate dehydratase

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Reaction

Synonyms

(S)-NAD(P)HX dehydratase, ADP-dependent (S)-NAD(P)H-hydrate dehydratase, ADP/ATP-dependent NAD(P)H-hydrate dehydratase, At5g19150, ATP-dependent H4NAD(P)OH dehydratase, ATP-dependent NNRD, BSU38720, Carkd, More, NAD(P)H-hydrate dehydratase, NAD(P)HX dehydratase, NAD(P)X dehydratase, NAXD, NNRD, reduced nicotinamide adenine dinucleotide hydrate dehydratase, YKL151C, YxkO

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.93 ATP-dependent NAD(P)H-hydrate dehydratase

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Results	in table
3761	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
8	Cofactor
12	Disease/ Diagnostics
1	Expression
37	General Information
4	kcat/KM [mM/s]
4	KM Value [mM]
30	Localization
6	Metals/Ions
4	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
22	Organism
2	Pathway
31	PDB ID
4	pH Optimum
1	pH Range
2	Posttranslational Modification
6	Protein Variants
4	Purification (Commentary)
2	Reaction
1	Reaction Type
16	Reference
23	Source Tissue
37	Substrates and Products (Substrate)
5	Subunits
42	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
4	Turnover Number [1/s]

Purification

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Purification on EC 4.2.1.93 - ATP-dependent NAD(P)H-hydrate dehydratase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

native enzyme from rat liver mitochondria by anion exchange chromatography, solubilization with 0.1% Triton X-100, desalting gel filtration, and Blue Trisacryl affinity chromatography

Rattus norvegicus

D4AAT7

747020

preparation of mesophyll protoplasts from stable Arabidopsis thaliana transfection lines recombinantly expressing the enzyme, isolation of mitochondria from transgenic seedlings or protoplasts, recombinant C-terminally His6-tagged enzyme from Escherichia coli by ncikel affinity chromatography. No copurification with coexpressed NNRE or PDX3

Arabidopsis thaliana

Q94AF2

748172

recombinant C-terminally His6-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Arabidopsis thaliana

Q94AF2

730041

recombinant Myc-His-tagged mCarkd from HEK-293T cells by affinity chromatography

Mus musculus

Q9CZ42

747020