4.2.1.92: hydroperoxide dehydratase

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For detailed information about hydroperoxide dehydratase, go to the full flat file.

Word Map on EC 4.2.1.92

4.2.1.92

lipoxygenase

jasmonic

oxylipins

linolenic

12-oxo-phytodienoic

aroma

hevea

divinyl

epoxyalcohols

9-hydroperoxides

lyases

cyclopentenone

alpha-linolenic

13-hpot

13-lipoxygenase

wound-induced

octadecanoids

laticifer

alpha-ketols

aloxe3

12r-lox

13-hydroperoxy

e-2-hexenal

hepoxilins

homomalla

coronatine

homolytic

hexenal

heterolytic

gaeumannomyces

antarafacial

attenuata

plexaura

food industry

medicine

synthesis

Reaction

(9Z,11E,15Z)-(13S)-hydroperoxyoctadeca-9,11,15-trienoate

(9Z,15Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate

Synonyms

10S-hydroperoxide lyase, 13-HPL, 13-hydroperoxide lyase, 13-hydroperoxide-lyase, 13HPL, 13S-hydroperoxide-dependent peroxidase, 5,8-hydroperoxide isomerase, 8,11-hydroperoxide isomerase, 9-HPL, 9/13 hydroperoxide lyase, 9R-DOX-AOS, 9S-DOX-AOS, allene oxide synthase, allene oxide synthase-1, AOS, AOS1, AOS2, cAOS, catalase-related allene oxide synthase, CmHPL, Csa_7G075590, CsHPL, CYP74, CYP741, CYP742, CYP74A, CYP74B, CYP74B24, CYP74c, CYP74C13_GM, CYP74C13_MT, CYP74C1_CS, CYP74C2, CYP74C31, CYP74C4_ST, cytochrome P450 74A, EC 5.3.99.1, eLOX3, fatty acid 9-hydroperoxide lyase, fatty acid hydroperoxide lyase, fatty acid hydroperoxide-metabolizing enzyme, Fg-cat, FG02216.1, FGSG_02217, FOXB_01332, HPI, HPL, HPL1, HPLS, HPO lyase, HPO-lyase, hydroperoxide dehydrase, hydroperoxide isomerase, hydroperoxide lyase, hydroperoxide lyase 1, hydroperoxidehydrase, isomerase, hydroperoxide, KC525886, linoleate hydroperoxide isomerase, linoleic acid hydroperoxide isomerase, LOX, Mn-LO, More, MtrunA17_Chr4g0034141, Npun_R5469, OsAOS1, OsHPL3, OsHPL3/CYP74B2, RPP, rubber particle protein

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.1 Hydro-lyases

4.2.1.92 hydroperoxide dehydratase

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Results	in table
7	Activating Compound
76	AA Sequence
5	Application
2	CAS Registry Number
28	Cloned(Commentary)
7	Cofactor
3	Crystallization (Commentary)
34	Disease/ Diagnostics
9	Expression
70	General Information
2	General Stability
18	Inhibitors
8	kcat/KM [mM/s]
12	KM Value [mM]
21	Localization
5	Metals/Ions
9	Molecular Weight [Da]
81	Natural Substrates/ Products (Substrates)
80	Organism
5	Pathway
21	PDB ID
28	pH Optimum
3	pH Range
1	pH Stability
1	pI Value
2	Posttranslational Modification
39	Protein Variants
23	Purification (Commentary)
8	Reaction
1	Reaction Type
78	Reference
51	Source Tissue
3	Specific Activity [micromol/min/mg]
2	Storage Stability
163	Substrates and Products (Substrate)
12	Subunits
186	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
1	Temperature Range [°C]
4	Temperature Stability [°C]
50	Turnover Number [1/s]

Purification

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Purification on EC 4.2.1.92 - hydroperoxide dehydratase

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5 entries

AOS1 purified on Ni-NTA resin

Physcomitrium patens

A9S014, A9SNA2

702754

native enzyme partially from leaves by ammonium sulfate fractionation

Amaranthus tricolor

747291

Ni-NTA column chromatography

Arabidopsis thaliana

Q96242

700387

partial

Hordeum vulgare subsp. vulgare

648493

partially, isolation of thylakoid membranes

Spinacia oleracea

749036

recombinant His-tagged enzyme from Escherichia coli membranes by solubilization with Triton X-100 or dodecyl maltoside, the latter results in higher enzyme activity, followed by nickel affinity chromatography

Capsicum annuum

730885

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography

Cucumis melo

Q93XR3

747189

recombinant His-tagged full-length and truncated enzymes from Escherichia coli strain M15 by cobalt affinity chromatography

Olea europaea

G8XQN2

746792, 746794

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography

Cucumis sativus

A0A0A0K2U8, U5YTM6

747189

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography

Medicago truncatula

Q7X9B3

747189

recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography

2 entries

recombinant His-tagged wild-type enzyme from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography

Glycine max

M4WFN0

747189

recombinant His6-tagged enzyme from Yarrowia lipolytica strain JMY 861 by solubilization by Triton X-100, nickel affinity chromatography, and ultrafiltration

Capsicum annuum

Q39443

747673

recombinant N- or C-terminal His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis

Nostoc punctiforme

B2J5P3

730040

solubilization of the enzyme from plant leaves by 1% Tween-20

2 entries