a cDNA encoding for olive HPL of Leccino variety is isolated from black olive fruits, cloned in pQE-30 expression vector, and expressed as His-tagged proteins in Escherichia coli strain M15. In order to improve the enzyme solubility, the chloroplast transit peptide is deleted
a unique cDNA fragment is isolated by suppressive subtractive hybridization (SSH) from a tea plant subjected to herbivory by tea geometrid Ectropis obliqua, a full-length cDNA is acquired by RACE, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), quantitative RT-PCR expression analysis
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami(DE3)pLysS B
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta-gami(DE3)pLysS B
functional recombinant expression of His6-tagged enzyme in Yarrowia lipolytica strain JMY 861, the enzyme appears after 24 h cultivation at the end of the exponential phase when the yeast is grown on a culture medium containing 10 g/l of olive oil as the sole carbon source, enzyme production method optimization, overview
gene 13-hpl, sequence comparisons, functional expression in Escherichia coli partly in inculsion bodies, fusion of the maltose binding protein malE to the 5'-truncated 13-hpl gene improves functional expression to some extent
gene ATEG_02036, sequence comparisons with other enzymes, cloning and functional expression of the enzyme with V5 epitope and His6-tag from plasmid pIZ/V5-His in Spodoptera frugiperda Sf9 cells and in Escherichia coli
gene CmHPL, expression in Nicotiana benthamiana leaves via infiltration method with a aviral vector, real-time PCR expression analysis over 10 days after infiltration with increase of C-9 aldehyde production, overview
gene CYP74B24, three HPL-related genes from Camellia sinensis are identified via RNA-sequencing in silico, gene CYP74B24 encodes a functional tea HPL enzyme, recombinant expression of CYP74B24 protein in Escherichia coli
gene FG02216.1, transient transfection in HeLa cells using VTF-7, a recombinant vaccinia virus containing the T7 RNA polymerase gene, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PgAOS1, phylogenetic analysis, quantitative enzyme expression analysis in different cultivars with and without infection by Sclerospora graminicola
genetic mapping and quantitative real-time PCR expression analysis of HPL1 in plants of diverse accessions, e.g. accessions C24 and Ler display large difference in HPL1 expression, overview. Transformation of accession Col-0 plants with the C24 HPL1 allele under transcriptional regulation of the 35S promoter revealing a strong negative correlation between HPL1 expression and arabidopside accumulation after tissue damage. A quantitative trait loci analysis of an F2 population created from a cross between C24 and Col-0 locates a region on chromosome four strongly linked to the capacity to form arabidopsides
whole ORF of AOS1 amplified and ligated into cloning vector pBluescript SK II(+), sequenced and ligated int vector pET23a and overexpressed in Escherichia coli BL21