Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.1.92: hydroperoxide dehydratase

This is an abbreviated version!
For detailed information about hydroperoxide dehydratase, go to the full flat file.

Word Map on EC 4.2.1.92

Reaction

(9Z,11E,15Z)-(13S)-hydroperoxyoctadeca-9,11,15-trienoate
=
(9Z,15Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate
 +
H2O

Synonyms

10S-hydroperoxide lyase, 13-HPL, 13-hydroperoxide lyase, 13-hydroperoxide-lyase, 13HPL, 13S-hydroperoxide-dependent peroxidase, 5,8-hydroperoxide isomerase, 8,11-hydroperoxide isomerase, 9-HPL, 9/13 hydroperoxide lyase, 9R-DOX-AOS, 9S-DOX-AOS, allene oxide synthase, allene oxide synthase-1, AOS, AOS1, AOS2, cAOS, catalase-related allene oxide synthase, CmHPL, Csa_7G075590, CsHPL, CYP74, CYP741, CYP742, CYP74A, CYP74B, CYP74B24, CYP74c, CYP74C13_GM, CYP74C13_MT, CYP74C1_CS, CYP74C2, CYP74C31, CYP74C4_ST, cytochrome P450 74A, EC 5.3.99.1, eLOX3, fatty acid 9-hydroperoxide lyase, fatty acid hydroperoxide lyase, fatty acid hydroperoxide-metabolizing enzyme, Fg-cat, FG02216.1, FGSG_02217, FOXB_01332, HPI, HPL, HPL1, HPLS, HPO lyase, HPO-lyase, hydroperoxide dehydrase, hydroperoxide isomerase, hydroperoxide lyase, hydroperoxide lyase 1, hydroperoxidehydrase, isomerase, hydroperoxide, KC525886, linoleate hydroperoxide isomerase, linoleic acid hydroperoxide isomerase, LOX, Mn-LO, More, MtrunA17_Chr4g0034141, Npun_R5469, OsAOS1, OsHPL3, OsHPL3/CYP74B2, RPP, rubber particle protein

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.92 hydroperoxide dehydratase

Cloned

Cloned on EC 4.2.1.92 - hydroperoxide dehydratase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a cDNA encoding for olive HPL of Leccino variety is isolated from black olive fruits, cloned in pQE-30 expression vector, and expressed as His-tagged proteins in Escherichia coli strain M15. In order to improve the enzyme solubility, the chloroplast transit peptide is deleted
a unique cDNA fragment is isolated by suppressive subtractive hybridization (SSH) from a tea plant subjected to herbivory by tea geometrid Ectropis obliqua, a full-length cDNA is acquired by RACE, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), quantitative RT-PCR expression analysis
-
AOS2 cloned and overexpressed in Escherichia coli
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami(DE3)pLysS B
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta-gami(DE3)pLysS B
CYP74C1_CS gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS
CYP74C4_ST gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS
expressed in Escherichia coli BL21(DE3) cells
expression in Pichia pastoris
functional recombinant expression of His6-tagged enzyme in Yarrowia lipolytica strain JMY 861, the enzyme appears after 24 h cultivation at the end of the exponential phase when the yeast is grown on a culture medium containing 10 g/l of olive oil as the sole carbon source, enzyme production method optimization, overview
gene 13-hpl, sequence comparisons, functional expression in Escherichia coli partly in inculsion bodies, fusion of the maltose binding protein malE to the 5'-truncated 13-hpl gene improves functional expression to some extent
-
gene ATEG_02036, sequence comparisons with other enzymes, cloning and functional expression of the enzyme with V5 epitope and His6-tag from plasmid pIZ/V5-His in Spodoptera frugiperda Sf9 cells and in Escherichia coli
gene CmHPL, expression in Nicotiana benthamiana leaves via infiltration method with a aviral vector, real-time PCR expression analysis over 10 days after infiltration with increase of C-9 aldehyde production, overview
-
gene CYP74B24, three HPL-related genes from Camellia sinensis are identified via RNA-sequencing in silico, gene CYP74B24 encodes a functional tea HPL enzyme, recombinant expression of CYP74B24 protein in Escherichia coli
gene FG02216.1, transient transfection in HeLa cells using VTF-7, a recombinant vaccinia virus containing the T7 RNA polymerase gene, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene FOXB_01332, phylogenetic tree, expression of wild-type and mutant enzymes
gene HPLCl, overexpression in Saccharomyces cerevisiae strain BY4743 isogenic
-
gene Npun_R5468, expression of the enzyme with N- or C-terminal His6 tags in Escherichia coli strain BL21(DE3)
gene OsHPL3, DNA and amino acid sequence determination, analysis, and comparison
-
gene PgAOS1, phylogenetic analysis, quantitative enzyme expression analysis in different cultivars with and without infection by Sclerospora graminicola
-
genetic mapping and quantitative real-time PCR expression analysis of HPL1 in plants of diverse accessions, e.g. accessions C24 and Ler display large difference in HPL1 expression, overview. Transformation of accession Col-0 plants with the C24 HPL1 allele under transcriptional regulation of the 35S promoter revealing a strong negative correlation between HPL1 expression and arabidopside accumulation after tissue damage. A quantitative trait loci analysis of an F2 population created from a cross between C24 and Col-0 locates a region on chromosome four strongly linked to the capacity to form arabidopsides
overexpression of His-tagged enzyme in Escherichia coli membranes
-
recombinant expression of His-tagged full-length enzyme or matured enzyme with truncated chloroplast transit peptide in Escherichia coli strain M15
semiquantitative RT-PCR HPL expression analysis
sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
whole ORF of AOS1 amplified and ligated into cloning vector pBluescript SK II(+), sequenced and ligated int vector pET23a and overexpressed in Escherichia coli BL21