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4.2.1.91: arogenate dehydratase

This is an abbreviated version!
For detailed information about arogenate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.91

Reaction

L-arogenate
=
L-phenylalanine
 +
H2O
 +
CO2

Synonyms

AdT, ADT/PDT, ADT1, ADT2, ADT3, ADT4, ADT5, ADT6, AGN dehydratase, arogenate dehydratase, arogenate dehydratase isoform 1, arogenate dehydratase isoform 2, arogenate dehydratase isoform 3, arogenate dehydratase isoform 4, arogenate dehydratase isoform 5, arogenate dehydratase isoform 6, arogenate dehydratase/prephenate dehydratase, arogenate dehydratase1, arogenate dehydratase2, arogenate dehydratase3, At1g08250, At1g11790, At2g27820, At3g07630, At3g44720, At5g22630, AtADT1, AtADT2, AtADT3, AtADT4, AtADT5, AtADT6, carboxycyclohexadienyl dehydratase, CDT, cyclohexadienyl dehydratase, dehydratase ADT3, F25C20.4 protein, Mtr1, prephenate dehydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.91 arogenate dehydratase

Cloned

Cloned on EC 4.2.1.91 - arogenate dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtADT1, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview
AtADT2, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview
AtADT3, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview
AtADT4, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview
AtADT5, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview
AtADT6, DNA and amino acid sequence determination and analysis, sequence comparison, complementation of the Saccharomyces cerevisiae pha2 mutant strain YNL316c, which lacks PDT activity and cannot grow in the absence of exogenous Phe by the six ADTs from Arabidopsis thaliana: AtADT2 readily recovers the pha2 phenotype after about 6 days growth at 30°C, while AtADT1 requires about 13 days to show visible growth. By contrast, AtADT6, with lowest PDT activity, and AtADT3-5, with no PDT activity, are unable to recover the phenotype, overview
coding regions corresponding to mature ADT protein (lacking the N-terminal plastid transit peptides) subcloned into the expression vector pET-28a, which contains an N-terminal 6xHis tag. Recombinant protein expressed in Escherichia coli Rosetta cells
expression in Escherichia coli
gene ADT1, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT1 in Arabidopsis thaliana
gene ADT2, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT2 in Arabidopsis thaliana
gene ADT3, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT3 in Arabidopsis thaliana
gene ADT4, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT4 in Arabidopsis thaliana
gene ADT5, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. CFP-tagged ADT5 is co-infiltrated with a YFP fusion to the nuclear marker NUP1, a component of the nuclear pore complex in Arabidopsis thaliana that has previously been shown to localize to the nuclear membrane. Transient expression pf CFP-tagged ADT5 in Arabidopsis thaliana
gene ADT6, recombinant expression of C-terminally CFP-tagged enzyme in Nicotiana benthamiana under control of the CaMV 35S promoter. Transient expression pf CFP-tagged ADT6 in Arabidopsis thaliana
genes adt1-adt6, complementation of the adt5 KO line with ADT5 gene expression under the control of its native promoter or the cauliflower mosaic virus 35S promoter
-
overexpression of the mutant gene in rice calli
-
pheC cloned and expressed by functional complementation of a pheA auxotroph of Escherichia coli