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4.2.1.90: L-rhamnonate dehydratase

This is an abbreviated version!
For detailed information about L-rhamnonate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.90

Reaction

L-Rhamnonate
=
2-dehydro-3-deoxy-L-rhamnonate
 +
H2O

Synonyms

dehydratase, L-rhamnonate, LRA3, Lra3p, LraC, More, PICST_50672, RhamD, YfaW

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.90 L-rhamnonate dehydratase

Cloned

Cloned on EC 4.2.1.90 - L-rhamnonate dehydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene LRA3, functional expression in Escherichia coli
gene LRA3, genetic organization, L-rhamnose catabolism genes RHA1, LRA2, LRA3 and LRA4 are organized in a chromosomal gene cluster in Scheffersomyces stipitis. In Scheffersomyces stipitis, another transcription factor than TRC1 is found in the L-rhamnose cluster, FST14. This transcription factor is more up-regulated than TRC1 on L-rhamnose and it might be co-regulating the pectinolytic genes together with TRC1
gene lraC, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae
gene lraC, DNA and amino acid sequencedetermination and analysis, expression in Saccharomyces cerevisiae
-
gene lraC, genetic organization, genes lraA, lraB and lraC, as well as the L-rhamnose regulator RhaR are organized in a cluster
-
gene yfaW, expression of the His6- or His10-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
the genes for L-rhamnose catabolism RHA1, LRA2, LRA3 and LRA4 but not LADH are clustered, genetic organization, phylogenetic analysis, overview. Functional expression of gene LRA3 in Saccharomyces cerevisiae with a strong constitutive promoter. C-terminally his-tagged protein shows no activity and the activity of the N-terminally tagged protein is reduced by about 95%, so the enzyme is expressed without tagand not purified