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4.2.1.81: D(-)-tartrate dehydratase

This is an abbreviated version!
For detailed information about D(-)-tartrate dehydratase, go to the full flat file.

Word Map on EC 4.2.1.81

Reaction

(S,S)-Tartrate
=
oxaloacetate
+
H2O

Synonyms

bll6730, D-tartrate dehydratase, dehydratase, D-tartrate, TarD

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.81 D(-)-tartrate dehydratase

Engineering

Engineering on EC 4.2.1.81 - D(-)-tartrate dehydratase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H322A
-
kcatKm is 193000fold lower than wild-type value
H322N
-
kcatKm is 607fold lower than wild-type value
H322Q
-
kcatKm is 340000fold lower than wild-type value
K102A
-
inactive mutant protein
K102M
-
inactive mutant protein
K184A
-
inactive mutant enzyme
K184R
-
reduced activity. kcat/Km is 500fold lower than wild-type value
additional information
-
selection of strains for constitutive production of enzyme for selective cleavage of racemic tartaric acid and quantitative detection of D(-)-tartrate