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4.2.1.78: (S)-norcoclaurine synthase

This is an abbreviated version!
For detailed information about (S)-norcoclaurine synthase, go to the full flat file.

Word Map on EC 4.2.1.78

Reaction

4-hydroxyphenylacetaldehyde
+
dopamine
=
(S)-norcoclaurine
 +
H2O

Synonyms

(S)-norcoclaurine synthase, (S)-Norlaudanosoline synthase, AmNCS1, AmNCS2, CCHNCS2, CjNCS1, CjPR10A, CsNCS, More, NCS, NCS1, NCS2, NDONCS3, norcoclaurine synthase, PbNCS, PR10A, PSONCS1, PSONCS2, S-norcoclaurine synthase, Synthase, (S)-norlaudanosoline, TFLNCSDELTA25, TfNCS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.78 (S)-norcoclaurine synthase

Engineering

Engineering on EC 4.2.1.78 - (S)-norcoclaurine synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A79F
improved turnover of ketones, increase in side-chain steric interactions
A79I
improved turnover of ketones, increase in side-chain steric interactions
E110A
site-directed mutagenesis, lower but significant activity
K122A
site-directed mutagenesis, lower but significant activity
Y108A
site-directed mutagenesis abolishes the stereoselective synthesis of (S)-norcoclaurine
Y108F
site-directed mutagenesis, reduced activity
additional information
-
virus-induced gene silencing of NCS in opium poppy profoundly reduces alkaloid levels compared to controls, a mixed culture of Agrobacterium tumefaciens harboring either pTRV2-PsNCS or pTRV1 is infiltrated into opium poppy seedlings tow weeks after germination. Levels of the five major alkaloids in opium poppy latex, i.e. morphine, codeine, thebaine, noscapine, and papaveine, are reduced by 75-82% in plants infiltrated with Agrobacterium tumefaciens harboring pTRV2-PsNCS