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4.2.1.75: uroporphyrinogen-III synthase

This is an abbreviated version!
For detailed information about uroporphyrinogen-III synthase, go to the full flat file.

Word Map on EC 4.2.1.75

Reaction

Hydroxymethylbilane
=
uroporphyrinogen III
 +
H2O

Synonyms

CobA/HemD, Hydroxymethylbilane hydrolyase [cyclizing], hydroxymethylbilane hydrolyase, cyclizing, Isomerase, uroporphyrinogen, Porphobilinogenase, Synthase, uroporphyrinogen III co-, U3S, URO synthase, uro'gen III synthase, URO-synthase, UROIIIS, Uroporphyrinogen III co-synthase, Uroporphyrinogen III cosynthase, Uroporphyrinogen III synthase, Uroporphyrinogen isomerase, Uroporphyrinogen-III cosynthase, Uroporphyrinogen-III cosynthetase, uroporphyrinogen-III-synthase, UROS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.75 uroporphyrinogen-III synthase

Cloned

Cloned on EC 4.2.1.75 - uroporphyrinogen-III synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes
expressed in Escherichia coli C41(DE3) cells
expression in Escherichia coli
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression of His-tagged wild-type and mutant enzymes in Escherichia coli
-
expression of N-terminally His-tagged enzyme in Escherichia coli strain strain BL21(DE3)
expression of the N-terminal His-tagged wild-type and mutant enzymes in Escherichia coli BL21(DE3)
expression of wild-type UROIIIS and 25 point mutants in Escherichia coli
-
gene cobA/hemD encoding the bifunctional uroporphyrinogen III synthase/methyltransferase, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21(DE3). Unsuccessful expressionin Lactococcus lactis strain NZ9000
gene HEM4, library screening and complementation studies, sequence comparisons, expression of the enzyme in the knockout Saccharomyces cerevisiae S150-2B strain, overvexpression of the His-tagged enzyme in Escherichia coli BL21(DE3) cells
gene pspto_0129, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3). The selenomethionine-substituted derivative is produced by expression in an Escherichia coli methionine auxotrophic strain B834(DE3)
overexpression as N-terminally his-tagged enzyme in Escherichia coli
-
overproduction
-
semi-quantitative real-time PCR analysis of ubiquitous and erythroid-specific UROS mRNAs, expression analysis
-
stably expression of GFP-tagged UROIIIS-WT or UROIIIS-C73R proteins in MLP29cell lines, reduced expression levels of C73R-UROIIIS in the mammalian cells
-